AUTHOR=Liao Hong , Li Qian , Chen Yulei , Tang Jiaye , Mou Borui , Lu Fujia , Feng Peng , Li Wei , Li Jialian , Fu Chun , Long Wencong , Xiao Ximeng , Han Xuebing , Xin Wenli , Yang Fengxuan , Ma Menggen , Liu Beidong , Yang Yaojun , Wang Hanyu 

TITLE=Genome-wide identification of resistance genes and response mechanism analysis of key gene knockout strain to catechol in Saccharomyces cerevisiae

JOURNAL=Frontiers in Microbiology

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1364425

DOI=10.3389/fmicb.2024.1364425

ISSN=1664-302X

ABSTRACT=<p>Engineering <italic>Saccharomyces cerevisiae</italic> for biodegradation and transformation of industrial toxic substances such as catechol (CA) has received widespread attention, but the low tolerance of <italic>S. cerevisiae</italic> to CA has limited its development. The exploration and modification of genes or pathways related to CA tolerance in <italic>S. cerevisiae</italic> is an effective way to further improve the utilization efficiency of CA. This study identified 36 genes associated with CA tolerance in <italic>S. cerevisiae</italic> through genome-wide identification and bioinformatics analysis and the <italic>ERG6</italic> knockout strain (<italic>ERG6</italic>Δ) is the most sensitive to CA. Based on the omics analysis of <italic>ERG6</italic>Δ under CA stress, it was found that <italic>ERG6</italic> knockout affects pathways such as intrinsic component of membrane and pentose phosphate pathway. In addition, the study revealed that 29 genes related to the cell wall-membrane system were up-regulated by more than twice, NADPH and NADP<sup>+</sup> were increased by 2.48 and 4.41 times respectively, and spermidine and spermine were increased by 2.85 and 2.14 times, respectively, in <italic>ERG6</italic>Δ. Overall, the response of cell wall-membrane system, the accumulation of spermidine and NADPH, as well as the increased levels of metabolites in pentose phosphate pathway are important findings in improving the CA resistance. This study provides a theoretical basis for improving the tolerance of strains to CA and reducing the damage caused by CA to the ecological environment and human health.</p>