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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Virology
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1360098
This article is part of the Research Topic Interferon response against viral infections View all 6 articles

Isolation of Limosilactobacillus mucosae G01 with Inhibitory Effects on Porcine Epidemic Diarrhea Virus in Vitro from Bama Pig Gastroenteritis

Provisionally accepted
Bin Zhang Bin Zhang 1,2Haiyan Shen Haiyan Shen 1Hongchao Gou Hongchao Gou 1Wuri Nile Wuri Nile 1,2Chunhong Zhang Chunhong Zhang 1Zhicheng Liu Zhicheng Liu 1,2Haiyan HE Haiyan HE 1,2Jingjing Nie Jingjing Nie 1Yunzhi Qv Yunzhi Qv 1Letu Geri Letu Geri 2*Jianfeng Zhang Jianfeng Zhang 1*
  • 1 Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, China
  • 2 College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia Autonomous Region, China

The final, formatted version of the article will be published soon.

    Porcine epidemic diarrhea virus (PEDV) is responsible for causing fatal watery diarrhea in piglets, resulting in significant economic losses within the pig farming industry. Although vaccination is currently employed as a preventive measure, certain vaccines do not provide complete protection against PEDV field strains. Probiotics present a promising alternative due to their ability to regulate intestinal flora, enhance host immunity, and improve resistance against pathogenic microorganisms.We isolated six lactic acid bacteria (LAB) from the fecal microorganisms of Bama pigs, compared to Limosilactobacillus mucosae DSM13345 of the same genus in which Limosilactobacillus mucosae G01 (L.mucosae G01) proved to have a potent anti-PEDV effect. In a comprehensive manner, L.mucosae G01 significantly augmented the phosphorylation of IRF3 in IPEC-J2 cells, resulting in the induction of interferons (IFN α, IFN β, IFN λ1, and IFN λ3) and subsequent upregulation of interferon-stimulated genes (ISGs) (MX1, MX2, OAS1, and ZAP) in a dose-dependent fashion, consequently leading to the mitigation of PEDV replication. These findings underscore the promising prospects of L.mucosae G01 as a naturally derived substitute for combating PEDV and other enteric coronavirus infections.

    Keywords: PEDV, Antiviral activity, L.mucosae G01, IFN, Bama pig

    Received: 22 Dec 2023; Accepted: 25 Jun 2024.

    Copyright: © 2024 Zhang, Shen, Gou, Nile, Zhang, Liu, HE, Nie, Qv, Geri and Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Letu Geri, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, 010000, Inner Mongolia Autonomous Region, China
    Jianfeng Zhang, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, China

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