AUTHOR=Lu Jinjing , Gong Xiaoying , Zhang Chenlu , Yang Tengfei , Pei Dongmei 

TITLE=A multi-omics approach to investigate characteristics of gut microbiota and metabolites in hypertension and diabetic nephropathy SPF rat models

JOURNAL=Frontiers in Microbiology

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1356176

DOI=10.3389/fmicb.2024.1356176

ISSN=1664-302X

ABSTRACT=<sec><title>Background</title><p>Imbalance in intestinal microbiota caused by microbial species and proportions or metabolites derived from microbes are associated with hypertension, as well as diabetic nephropathy. However, the involvement of the intestinal microbiota and metabolites in hypertension and diabetic nephropathy comorbidities (HDN) remains to be elucidated.</p></sec><sec><title>Methods</title><p>We investigated the effects of intestinal microbiota on HDN in a rat model and determined the abundance of the intestinal microbiota using 16S rRNA sequencing. Changes in fecal and serum metabolites were analyzed using ultra-high-performance liquid chromatography-mass spectrometry.</p></sec><sec><title>Results</title><p>The results showed abundance of <italic>Proteobacteria</italic> and <italic>Verrucomicrobia</italic> was substantially higher, whereas that of <italic>Bacteroidetes</italic> was significant lower in the HDN group than in the sham group. <italic>Akkermansia, Bacteroides, Blautia, Turicibacter, Lactobacillus, Romboutsia, and Fusicatenibacter</italic> were the most abundant, and <italic>Prevotella, Lachnospiraceae_NK4A136_group</italic>, and <italic>Prevotella_9</italic> were the least abundant in the HDN group. Further analysis with bile acid metabolites in serum showed that <italic>Blautia</italic> was negatively correlated with taurochenodeoxycholic acid, taurocholic acid, positively correlated with cholic acid and glycocholic acid in serum.</p></sec><sec><title>Conclusions</title><p>These findings suggest that the gut microbiota and metabolites in feces and serum substantially differed between the HDN and sham groups. The F/B ratio was higher in the HDN group than in the sham group. <italic>Blautia</italic> is potentially associated with HDN that correlated with differentially expressed bile acid metabolites, which might regulate the pathogenesis of HDN <italic>via</italic> the microorganism–gut–metabolite axis.</p></sec>