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ORIGINAL RESEARCH article
Front. Microbiol.
Sec. Microbiological Chemistry and Geomicrobiology
Volume 15 - 2024 |
doi: 10.3389/fmicb.2024.1349152
This article is part of the Research Topic Unlocking the Value of Wastewater: Innovative Biotechnologies and Bioprocesses for Resource Recovery in a Circular Economy Approach View all 7 articles
Nitrogen removal capability and mechanism of a novel lowtemperature-tolerant simultaneous nitrification-denitrification bacterium Acinetobacter kyonggiensis AKD4
Provisionally accepted- 1 Zhejiang Agriculture and Forestry University, Hangzhou, Zhejiang Province, China
- 2 Zhejiang Sunda Public Environmental Protection Co., LTD,, Hangzhou,Zhejiang, China
A low-temperature-tolerant simultaneous nitrification-denitrification bacterial strain of Acinetobacter kyonggiensis (AKD4) was identified. It showed high efficiency in total nitrogen (TN) removal (92.45% at 10 °C and 87.51% at 30 °C), indicating its excellent low-temperature tolerance. Transcriptomic analysis revealed possible metabolic mechanisms under low-temperature stress. Genes involved in cell growth, including ATP synthase (atpADGH), amino acid (glyA, dctA, and ilvE), and TCA cycle metabolism (gltA, fumC, and mdh) were remarkably upregulated from 1.05-to 3.44-fold at 10 °C, suggesting that their actions enhance survivability at low temperatures. The expression levels of genes associated with nitrogen assimilation (glnAE, gltBD, and gdhA), nitrogen metabolism regulation (ntrC, glnB, and glnD), and denitrification processes (napA) were increased from 1.01-to 4.38-fold at 10°C, which might have contributed to the bacterium's highly efficient nitrogen removal performance at low temperatures. Overall, this study offers valuable insights into transcriptome, and enhances the comprehension of the low-temperature-tolerant mechanism of simultaneous nitrification and denitrification processes.
Keywords: Simultaneous nitrification and denitrification, Low temperature, Transcriptomics, cell growth, nitrogen metabolism
Received: 04 Dec 2023; Accepted: 30 Aug 2024.
Copyright: © 2024 Mao, Zhao, Li, Qin, Wu, Xu, Jin and Zheng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Jiwei Mao, Zhejiang Agriculture and Forestry University, Hangzhou, 311300, Zhejiang Province, China
Ruojin Zhao, Zhejiang Sunda Public Environmental Protection Co., LTD,, Hangzhou,Zhejiang, China
Yiyi Li, Zhejiang Sunda Public Environmental Protection Co., LTD,, Hangzhou,Zhejiang, China
Wenpan Qin, Zhejiang Sunda Public Environmental Protection Co., LTD,, Hangzhou,Zhejiang, China
Shengchun Wu, Zhejiang Sunda Public Environmental Protection Co., LTD,, Hangzhou,Zhejiang, China
Weiping Xu, Zhejiang Sunda Public Environmental Protection Co., LTD,, Hangzhou,Zhejiang, China
Peng Jin, Zhejiang Sunda Public Environmental Protection Co., LTD,, Hangzhou,Zhejiang, China
Zhanwang Zheng, Zhejiang Agriculture and Forestry University, Hangzhou, 311300, Zhejiang Province, China
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