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ORIGINAL RESEARCH article

Front. Microbiol.
Sec. Microbiological Chemistry and Geomicrobiology
Volume 15 - 2024 | doi: 10.3389/fmicb.2024.1349152
This article is part of the Research Topic Unlocking the Value of Wastewater: Innovative Biotechnologies and Bioprocesses for Resource Recovery in a Circular Economy Approach View all 7 articles

Nitrogen removal capability and mechanism of a novel lowtemperature-tolerant simultaneous nitrification-denitrification bacterium Acinetobacter kyonggiensis AKD4

Provisionally accepted
Jiwei Mao Jiwei Mao 1*Ruojin Zhao Ruojin Zhao 2*Yiyi Li Yiyi Li 2*Wenpan Qin Wenpan Qin 2*Shengchun Wu Shengchun Wu 2*Weiping Xu Weiping Xu 2*Peng Jin Peng Jin 2*Zhanwang Zheng Zhanwang Zheng 1*
  • 1 Zhejiang Agriculture and Forestry University, Hangzhou, Zhejiang Province, China
  • 2 Zhejiang Sunda Public Environmental Protection Co., LTD,, Hangzhou，Zhejiang, China

The final, formatted version of the article will be published soon.

    A low-temperature-tolerant simultaneous nitrification-denitrification bacterial strain of Acinetobacter kyonggiensis (AKD4) was identified. It showed high efficiency in total nitrogen (TN) removal (92.45% at 10 °C and 87.51% at 30 °C), indicating its excellent low-temperature tolerance. Transcriptomic analysis revealed possible metabolic mechanisms under low-temperature stress. Genes involved in cell growth, including ATP synthase (atpADGH), amino acid (glyA, dctA, and ilvE), and TCA cycle metabolism (gltA, fumC, and mdh) were remarkably upregulated from 1.05-to 3.44-fold at 10 °C, suggesting that their actions enhance survivability at low temperatures. The expression levels of genes associated with nitrogen assimilation (glnAE, gltBD, and gdhA), nitrogen metabolism regulation (ntrC, glnB, and glnD), and denitrification processes (napA) were increased from 1.01-to 4.38-fold at 10°C, which might have contributed to the bacterium's highly efficient nitrogen removal performance at low temperatures. Overall, this study offers valuable insights into transcriptome, and enhances the comprehension of the low-temperature-tolerant mechanism of simultaneous nitrification and denitrification processes.

    Keywords: Simultaneous nitrification and denitrification, Low temperature, Transcriptomics, cell growth, nitrogen metabolism

    Received: 04 Dec 2023; Accepted: 30 Aug 2024.

    Copyright: © 2024 Mao, Zhao, Li, Qin, Wu, Xu, Jin and Zheng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Jiwei Mao, Zhejiang Agriculture and Forestry University, Hangzhou, 311300, Zhejiang Province, China
    Ruojin Zhao, Zhejiang Sunda Public Environmental Protection Co., LTD,, Hangzhou，Zhejiang, China
    Yiyi Li, Zhejiang Sunda Public Environmental Protection Co., LTD,, Hangzhou，Zhejiang, China
    Wenpan Qin, Zhejiang Sunda Public Environmental Protection Co., LTD,, Hangzhou，Zhejiang, China
    Shengchun Wu, Zhejiang Sunda Public Environmental Protection Co., LTD,, Hangzhou，Zhejiang, China
    Weiping Xu, Zhejiang Sunda Public Environmental Protection Co., LTD,, Hangzhou，Zhejiang, China
    Peng Jin, Zhejiang Sunda Public Environmental Protection Co., LTD,, Hangzhou，Zhejiang, China
    Zhanwang Zheng, Zhejiang Agriculture and Forestry University, Hangzhou, 311300, Zhejiang Province, China

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