AUTHOR=Ning Zhe , Hu Bo , Sun Yuan-Yuan , Ding Jin-Feng , Han Xiang-Ying , Lu Xiao-Ling , Yin Zi-Fei , He Ying , Jiao Bing-Hua , Yu Hao-Bing , Liu Xiao-Yu 

TITLE=Eutypellaolides A–J, Sesquiterpene diversity expansion of the polar fungus Eutypella sp. D-1

JOURNAL=Frontiers in Microbiology

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1349151

DOI=10.3389/fmicb.2024.1349151

ISSN=1664-302X

ABSTRACT=<p>Eight new 12,8-eudesmanolide sesquiterpenes, eutypellaolides A–H (<bold>1</bold>–<bold>8</bold>), and two new eudesmane-type sesquiterpenes, eutypellaolides I–J (<bold>9</bold>–<bold>10</bold>), along with four known 12,8-eudesmanolide compounds <bold>11</bold>–<bold>14</bold>, were isolated from the culture extract of the polar fungus <italic>Eutypella</italic> sp. D-1 by one strain many compounds (OSMAC) approach. The structures of these compounds were determined through comprehensive spectroscopic data and experimental and calculated ECD analysis. Antibacterial, immunosuppressive, and PTP1B inhibition activities of these compounds were evaluated. Compounds <bold>1</bold> and <bold>11</bold> exhibited strong inhibitory activities against <italic>Bacillus subtilis</italic> and <italic>Staphylococcus aureus</italic>, with each showing an MIC value of 2 μg/mL. Compound <bold>9</bold> displayed weak immunosuppressive activity against ConA-induced T-cell proliferation with an inhibitory rate of 61.7% at a concentration of 19.8 μM. Compounds <bold>5</bold>, <bold>11</bold>, and <bold>14</bold> exhibited weak PTP1B inhibition activities with IC<sub>50</sub> values of 44.8, 43.2, and 49.5 μM, respectively.</p>