AUTHOR=Tong Li , Jun Ling , He Jiang , Min Yang , Guoqing Duan , Yuting Hu , Huaxing Zhou , Huan Wang , Tingshuang Pan 

TITLE=Differences in environmental microbial community responses under rice-crab co-culture and crab monoculture models under cyanobacterial bloom

JOURNAL=Frontiers in Microbiology

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1327520

DOI=10.3389/fmicb.2024.1327520

ISSN=1664-302X

ABSTRACT=<p>Cyanobacterial blooms (CBs) present significant challenges to Chinese mitten crab (CMC) culture, posing hazards to the aquatic microbial ecology. However, the current focus on the microbial ecological changes within the CMC culture system under the influence of CBs is somewhat insufficient. There’s an urgent need to analyze the microbial ecosystem of the CMC culture system under CBs. This study employed 16S rRNA gene amplicon sequencing to investigate the dynamics of the environmental microbial community in both the rice-crab co-culture (RC) and crab monoculture (CM) models. The results revealed that cyanobacteria reached high levels in the CM water in July, while they began to increase in the RC water in August. Notably, OTU147 (uncultured bacterium g_<italic>Planktothrix NIVA-CYA 15</italic>), identified as the dominant taxon associated with CBs, showed a significant linear relationship with TP, NO<sub>2</sub><sup>−</sup>-N, and the N:P ratio. TP, TN, NO<sub>2</sub><sup>−</sup>-N, and COD<sub>Mn</sub> had a more pronounced impact on the structure of bacterial communities and cyanobacterial taxa in the water. The bacterial community structure involved in carbon metabolism displayed temporal succession in the water. The co-occurrence network of the bacterial community primarily consisted of <italic>Chloroflexi</italic>, <italic>Proteobacteria</italic>, and <italic>Firnicutes</italic> in the sediment, and <italic>Actinobacteria</italic>, <italic>Proteobacteria</italic>, <italic>Chloroflexi</italic>, and <italic>Bacteroidota</italic> in the water. In contrast, the co-occurrence network included different peripheral species in the sediment and water. Keystone species were predominantly represented by OTU22 (<italic>uncultured actinobacterium g_ hgcI clade</italic>) and OTU12 (uncultured <italic>Opitutae bacterium</italic> g_ norank) in the RC water, and by OTU25 (<italic>uncultured bacterium g_ Limnohabitans</italic>) in the CM water. TP, TN, NO<sub>2</sub><sup>−</sup>-N, and COD<sub>Mn</sub> were identified as the primary environmental factors influencing these keystone taxa within the culture water. In conclusion, this study on the microbial ecology of the CMC culture system under the influence of CBs provides valuable insights that can be instrumental in subsequent management efforts.</p>