AUTHOR=Shang Fangjian , Jiang Xia , Wang Haobo , Guo Shang , Kang Shuo , Xu Bin , Wang Xin , Chen Shihao , Li Ning , Liu Bo , Zhao Zengren 

TITLE=Bifidobacterium longum suppresses colorectal cancer through the modulation of intestinal microbes and immune function

JOURNAL=Frontiers in Microbiology

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1327464

DOI=10.3389/fmicb.2024.1327464

ISSN=1664-302X

ABSTRACT=<p>Colorectal cancer (CRC), one of the most common malignancies in the world, urgently requires more treatment strategies. Although there has been much research on probiotics, limited research has been done in treating cancer. The purpose of this study was to investigate the role of <italic>Bifidobacterium longum</italic> (<italic>B. longum</italic>) in the prevention and treatment of CRC. Through Cell Counting Kit-8 and Colony Formation Assays, 8 h and a <italic>B. longum</italic> count of 1 × 10<sup>8</sup> CFU/ml were chosen as the best cocultivation conditions with CRC cells. The role of <italic>B. longum</italic> in inhibiting the progression of CRC cells was verified by a series of functional and immunofluorescence assays. For instance, <italic>in vivo</italic> assays have verified that <italic>B. longum</italic> could alleviate CRC progression. In addition, according to the results of <italic>in vivo</italic> assays and clinical statistical analysis, <italic>B. longum</italic> could reduce diarrhea symptoms. Mechanistically, by 16S and RNA sequencing, it was found that <italic>B. longum</italic> could affect the development of CRC by regulating the composition of gut microbes and enhancing immune function. The <italic>B. longum</italic> might inhibit the occurrence and development of CRC and relieve diarrhea symptoms by regulating intestinal microbes and immune function.</p>