AUTHOR=Yang Shiyue , Di Lodovico Eliana , Rupp Alina , Harms Hauke , Fricke Christian , Miltner Anja , Kästner Matthias , Maskow Thomas 

TITLE=Enhancing insights: exploring the information content of calorespirometric ratio in dynamic soil microbial growth processes through calorimetry

JOURNAL=Frontiers in Microbiology

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1321059

DOI=10.3389/fmicb.2024.1321059

ISSN=1664-302X

ABSTRACT=<p>Catalytic activity of microbial communities maintains the services and functions of soils. Microbial communities require energy and carbon for microbial growth, which they obtain by transforming organic matter (OM), oxidizing a fraction of it and transferring the electrons to various terminal acceptors. Quantifying the relations between matter and energy fluxes is possible when key parameters such as reaction enthalpy (<italic>∆</italic><sub>r</sub><italic>H</italic>), energy use efficiency (related to enthalpy) (EUE), carbon use efficiency (CUE), calorespirometric ratio (CR), carbon dioxide evolution rate (CER), and the apparent specific growth rate (<inline-formula><mml:math id="M1" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mrow><mml:msub><mml:mi>μ</mml:mi><mml:mrow><mml:mi mathvariant="normal">app</mml:mi></mml:mrow></mml:msub></mml:mrow></mml:math></inline-formula>) are known. However, the determination of these parameters suffers from unsatisfying accuracy at the technical (sample size, instrument sensitivity), experimental (sample aeration) and data processing levels thus affecting the precise quantification of relationships between carbon and energy fluxes. To address these questions under controlled conditions, we analyzed microbial turnover processes in a model soil amended using a readily metabolizable substrate (glucose) and three commercial isothermal microcalorimeters (MC-Cal/100P, TAM Air and TAM III) with different sample sizes meaning varying volume-related thermal detection limits (<italic>LOD</italic><sub><italic>v</italic></sub>) (0.05<inline-formula><mml:math id="M2" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mrow><mml:mo>−</mml:mo><mml:mn>1</mml:mn><mml:mspace width="thickmathspace" /></mml:mrow></mml:math></inline-formula>mW L<sup>−1</sup>). We conducted aeration experiments (aerated and un-aerated calorimetric ampoules) to investigate the influence of oxygen limitation and thermal perturbation on the measurement signal. We monitored the CER by measuring the additional heat caused by CO<sub>2</sub> absorption using a NaOH solution acting as a CO<sub>2</sub> trap. The range of errors associated with the calorimetrically derived <inline-formula><mml:math id="M3" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mrow><mml:msub><mml:mi>μ</mml:mi><mml:mrow><mml:mi mathvariant="normal">app</mml:mi></mml:mrow></mml:msub></mml:mrow></mml:math></inline-formula>, EUE, and CR was determined and compared with the requirements for quantifying CUE and the degree of anaerobicity (<inline-formula><mml:math id="M4" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mrow><mml:msub><mml:mi>η</mml:mi><mml:mi mathvariant="normal">A</mml:mi></mml:msub><mml:mo stretchy="false">)</mml:mo></mml:mrow></mml:math></inline-formula>. Calorimetrically derived <inline-formula><mml:math id="M5" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mrow><mml:msub><mml:mi>μ</mml:mi><mml:mrow><mml:mi mathvariant="normal">app</mml:mi></mml:mrow></mml:msub></mml:mrow></mml:math></inline-formula> and EUE were independent of the instrument used. However, instruments with a low <italic>LOD<sub>v</sub></italic> yielded the most accurate results. Opening and closing the ampoules for oxygen and CO<sub>2</sub> exchange did not significantly affect metabolic heats. However, regular opening during calorimetrically derived CER measurements caused significant measuring errors due to strong thermal perturbation of the measurement signal. Comparisons between experimentally determined CR, CUE,<inline-formula><mml:math id="M6" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mrow><mml:mspace width="thickmathspace" /><mml:msub><mml:mi>η</mml:mi><mml:mi mathvariant="normal">A</mml:mi></mml:msub></mml:mrow></mml:math></inline-formula>, and modeling indicate that the evaluation of CR should be performed with caution.</p>