AUTHOR=Liu Guangtian , Ma Xueping , Zhang Ruiqing , Lü Jufen , Zhou Pan , Liu Bofei , Liu Tao , Ren Hui , Liu Zhiguo , Li Zhenjun , Jiang Xuefeng 

TITLE=Epidemiological changes and molecular characteristics of Brucella strains in Ningxia, China

JOURNAL=Frontiers in Microbiology

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1320845

DOI=10.3389/fmicb.2024.1320845

ISSN=1664-302X

ABSTRACT=<sec id="sec1"><title>Objective</title><p>Human brucellosis causes serious public health concerns in Ningxia, China.</p></sec><sec id="sec2"><title>Methods</title><p>This study employed epidemiological, bacteriological, and multiple-locus variable-number tandem repeat analysis (MLVA) methods to conduct an epidemiological investigation, which is necessary for devising tailored control strategies.</p></sec><sec id="sec3"><title>Results</title><p>Between 1958 and 2022, 29,892 cases were reported, with an average annual number of cases and incidence of 467 and 7.1/100,000, respectively. The epidemic situation gradually worsened, with cases escalating from 26 cases in 2005 to 6,292 in 2022, with the incidence rate rising from 0.441 in 2005 to 86.83 in 2022. Geographically, the disease spread from a single affected county in 2004 to encompass all 22 counties in 2022. Yanchi County had the highest incidence, followed by the Hongsibao and Tongxin counties. These data suggest that <italic>Brucella</italic> infection has become a rampant regional concern in human brucellosis. Between 1958 and 2019, a total of 230 <italic>Brucella</italic> strains were identified across four studied hosts. These strains comprised four species with 12 biovars, including <italic>B. melitensis</italic> bv. 1, bv. 2, bv. 3, <italic>B. abortus</italic> bv. 1, bv. 3, bv. 4, bv. 5, bv. 6, bv. 7, <italic>B. suis</italic> bv. 1 and bv. 3, and <italic>B. canis</italic>. These data highlight the high species/biovars and host diversity of the <italic>Brucella</italic> population, posing a substantial challenge to brucellosis surveillance. There was an apparent transition from multiple species/biovars historically to the current dominance of a single species, <italic>B. melitensis</italic>, emphasizing the requirement for strengthening surveillance of <italic>B. melitensis</italic>. Genotypes 42 and 116, constituting 96.2% of the total number of genotypes, predominated in panel 1 and <italic>MLVA-11</italic>, indicating that all strains belong to the East Mediterranean lineage. <italic>MLVA</italic> cluster analysis revealed persistent transmission of dominant circulating genotypes, presenting an epidemic pattern characterized primarily by epidemiologically related cases with a few sporadic cases. Strains in this study exhibited high genetic homogeneity with strains from the Northwest, and those from Kazakhstan and Mongolia.</p></sec><sec id="sec4"><title>Conclusion</title><p>The epidemic situation of human brucellosis has gradually worsened; the rampant epidemic of the disease has become a regional concern. The present study highlights that implementing the of targeted surveillance and intervention strategies is urge.</p></sec>