AUTHOR=Zang Yanqing , Ge Yinchen , Cao Yang , Tang Huacheng 

TITLE=Anti-diabetic effect of red quinoa polysaccharide on type 2 diabetic mellitus mice induced by streptozotocin and high-fat diet

JOURNAL=Frontiers in Microbiology

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1308866

DOI=10.3389/fmicb.2024.1308866

ISSN=1664-302X

ABSTRACT=<p>The purpose of this study was to explore the mechanism of red quinoa polysaccharide (RQP) in alleviating type 2 diabetes (T2D) through <italic>in vivo</italic> and <italic>in vitro</italic> experiments. Results of HPLC and FITR showed that RQP was a complex polysaccharide and contained more glucose, galactose and acarbose. <italic>In vitro</italic> experiments, RQP showed strong antioxidant capacity and inhibition on α-amylase and α-glucosidase. <italic>In vivo</italic> experiments, RQP was proved to induce a significant improvement of diabetes after 4 weeks of ingestion, including the abilities of lowering blood glucose, regulating lipid metabolism, anti-oxidation and promoting secretion of SCFAs. Furthermore, 16S rRNA study demonstrated that RQP transformed the intestinal microbiota composition in diabetic mice, decreased the abundance of <italic>norank_f_Muribaculaceae</italic> and <italic>Lachnospiraceae_NK4A136_group</italic>, and increased the relative abundance of <italic>Akkermansia</italic>, <italic>unclassified_f_Lachnospiraceae</italic>, <italic>norank_f_Eubacterium_coprostanoligenes_group</italic>, <italic>unclassified_f_Atopobiaceae</italic> and <italic>norank_f_Lachnospiraceae</italic>. The biosynthetic pathways, metabolic pathways and intestinal microbiome phenotypes in mice also changed accordingly. In conclusion, this study suggests that RQP can inhibit the development of diabetes by correcting the imbalance of intestinal flora.</p>