AUTHOR=Jiang Qiuyu , Zhao Lihua , Ban Zhibin , Zhang Bingkun 

TITLE=Different fat-to-fiber ratios by changing wheat inclusion level impact energy metabolism and microbial structure of broilers

JOURNAL=Frontiers in Microbiology

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1298262

DOI=10.3389/fmicb.2024.1298262

ISSN=1664-302X

ABSTRACT=<sec><title>Introduction</title><p>Dietary nutrient content is crucial for energy metabolism and development of gut microbiota. Herein, this study aimed to explore the effects of fat-to-fiber ratios on nutrient transporter, energy metabolism and gut microbiota when ingredients composition was altered.</p></sec><sec><title>Methods</title><p>A total of 240 as-hatched broiler chickens were randomly assigned into three groups including low fat-high dietary fiber (LF-HD), medium fat-medium dietary fiber (MF-MD) and high fat-low dietary fiber (HF-LD), with diets being iso-protein, and broilers were offered the same commercial diets from 21 to 42 d. The data were analyzed using one-way ANOVA of SPSS.</p></sec><sec><title>Results and Discussion</title><p>Results showed that HF-LD diet significantly increased glucose content and decreased triglyceride in serum of broilers (<italic>p</italic> &lt; 0.05). The mRNA abundance of jejunal gene involved in glucose transporter and tricarboxylic acid (TCA) cycle was significantly increased in broilers fed with HF-LD diets. Compared with LF-HD, HF-LD had a lower abundance of <italic>Anaerofilum</italic> and <italic>CHKCI001</italic>, and an increased proportion of beneficial bacteria such as <italic>Alistipes</italic>, <italic>Catenibacillus</italic>, <italic>Intestinimonas</italic>, <italic>Lactobacillus</italic>, and <italic>Peptococcus</italic> (<italic>p</italic> &lt; 0.05). Functional prediction of these microbial changes indicated that HF-LD diet drove caecal microbiota to participate in carbohydrate metabolism and TCA cycle (<italic>p</italic> &lt; 0.05). Dietary HF-LD-induced microbiota changes were positively correlated with growth performance of broilers (<italic>p</italic> &lt; 0.05). Therefore, HF-LD diet increased glucose transporters and energy metabolism in intestine and shaped microbial structure and metabolic pathways, which may benefit the growth performance of broilers.</p></sec>