AUTHOR=Gao Weiqi , Yan Ya , Guan Zhaobo , Zhang Jingmin , Chen Weihong 

TITLE=Effects of Bacillus coagulans TBC169 on gut microbiota and metabolites in gynecological laparoscopy patients

JOURNAL=Frontiers in Microbiology

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1284402

DOI=10.3389/fmicb.2024.1284402

ISSN=1664-302X

ABSTRACT=<sec id="sec1"><title>Objective</title><p>The primary objective of this study is to investigate the mechanism by which <italic>Bacillus coagulans</italic> TBC169 accelerates intestinal function recovery in patients who have undergone gynecological laparoscopic surgery, using metabolomics and gut microbiota analysis.</p></sec><sec id="sec2"><title>Methods</title><p>A total of 20 subjects were selected and randomly divided into two groups: the intervention group (<italic>n</italic> = 10) receiving <italic>Bacillus coagulans</italic> TBC169 Tablets (6 pills, 1.05 × 10<sup>8</sup> CFU), and the control group (<italic>n</italic> = 10) receiving placebos (6 pills). After the initial postoperative defecation, fecal samples were collected from each subject to analyze their gut microbiota and metabolic profiles by high-throughput <italic>16S</italic> rRNA gene sequencing analysis and untargeted metabonomic.</p></sec><sec id="sec3"><title>Results</title><p>There were no statistically significant differences observed in the α-diversity and β-diversity between the two groups; however, in the intervention group, there was a significant reduction in the relative abundance of <italic>unclassified_Enterobacteriaceae</italic> at the genus level. Furthermore, the control group showed increased levels of <italic>Holdemanella</italic> and <italic>Enterobacter</italic>, whereas the intervention group exhibited elevated levels of <italic>Intestinimonas</italic>. And administration of <italic>Bacillus coagulans</italic> TBC169 led to variations in 2 metabolic pathways: D-glutamine and D-glutamate metabolism, and arginine biosynthesis.</p></sec><sec id="sec4"><title>Conclusion</title><p>This study demonstrated that consuming <italic>Bacillus coagulans</italic> TBC169 after gynecological laparoscopic surgery might inhibit the proliferation of harmful <italic>Enterobacteriaceae</italic>; mainly influence 2 pathways including D-glutamine and D-glutamate metabolism, and arginine biosynthesis; and regulate metabolites related to immunity and intestinal motility; which can help regulate immune function, maintain intestinal balance, promote intestinal peristalsis, and thus accelerate the recovery of intestinal function.</p></sec>