AUTHOR=Flores-Oropeza Marco A. , Ochoa Sara A. , Cruz-Córdova Ariadnna , Chavez-Tepecano Rolando , Martínez-Peñafiel Eva , Rembao-Bojórquez Daniel , Zavala-Vega Sergio , Hernández-Castro Rigoberto , Flores-Encarnacion Marcos , Arellano-Galindo José , Vélez Daniel , Xicohtencatl-Cortes Juan 

TITLE=Comparative genomic analysis of uropathogenic Escherichia coli strains from women with recurrent urinary tract infection

JOURNAL=Frontiers in Microbiology

VOLUME=14

YEAR=2024

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1340427

DOI=10.3389/fmicb.2023.1340427

ISSN=1664-302X

ABSTRACT=<sec id="sec1"><title>Introduction</title><p>Recurrent urinary tract infections (RUTIs) caused by uropathogenic <italic>Escherichia coli</italic> are costly public health problems impacting patients’ quality of life.</p></sec><sec id="sec2"><title>Aim</title><p>In this work, a comparative genomics analysis of three clinical RUTI strains isolated from bladder biopsy specimens was performed.</p></sec><sec id="sec3"><title>Materials and methods</title><p>One hundred seventy-two whole genomes of urinary tract <italic>E. coli</italic> strains were selected from the NCBI database. The search for virulence factors, fitness genes, regions of interest, and genetic elements associated with resistance was manually carried out. The phenotypic characterization of antibiotic resistance, haemolysis, motility, and biofilm formation was performed. Moreover, adherence and invasion assays with human bladder HTB-5 cells, and transmission electron microscopy (TEM) were performed.</p></sec><sec id="sec4"><title>Results</title><p>The UTI-1_774U and UTI-3_455U/ST1193 strains were associated with the extraintestinal pathotypes, and the UTI-2_245U/ST295 strain was associated with the intestinal pathotype, according to a phylogenetic analysis of 172 <italic>E. coli</italic> urinary strains. The three RUTI strains were of clinical, epidemiological, and zoonotic relevance. Several resistance genes were found within the plasmids of these strains, and a multidrug resistance phenotype was revealed. Other virulence genes associated with CFT073 were not identified in the three RUTI strains (genes for type 1 and P fimbriae, haemolysin <italic>hlyA,</italic> and <italic>sat</italic> toxin). Quantitative adherence analysis showed that UTI-1_774U was significantly (<italic>p</italic> &lt; 0.0001) more adherent to human bladder HTB-5 cells. Quantitative invasion analysis showed that UTI-2_245U was significantly more invasive than the control strains. No haemolysis or biofilm activity was detected in the three RUTI strains. The TEM micrographs showed the presence of short and thin fimbriae only in the UTI-2_245U strain.</p></sec><sec id="sec5"><title>Conclusion</title><p>The high variability and genetic diversity of the RUTI strains indicate that are a mosaic of virulence, resistance, and fitness genes that could promote recurrence in susceptible patients.</p></sec>