AUTHOR=Kojima Daiki , Tanaka Shunsuke , Kurosaki Ayane , Zhiyu Xiong , Ito Masahiro TITLE=Isolation and Cs+ resistance mechanism of Escherichia coli strain ZX-1 JOURNAL=Frontiers in Microbiology VOLUME=14 YEAR=2024 URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1340033 DOI=10.3389/fmicb.2023.1340033 ISSN=1664-302X ABSTRACT=

This research aims to elucidate the physiological mechanisms behind the accidental acquisition of high-concentration cesium ions (Cs⁺) tolerance of Escherichia coli and apply this understanding to develop bioremediation technologies. Bacterial Cs⁺ resistance has attracted attention, but its physiological mechanism remains largely unknown and poorly understood. In a prior study, we identified the Cs⁺/H⁺ antiporter TS_CshA in Microbacterium sp. TS-1, resistant to high Cs⁺ concentrations, exhibits a low Cs⁺ affinity with a K_m value of 370 mM at pH 8.5. To enhance bioremediation efficacy, we conducted random mutagenesis of TS_cshA using Error-Prone PCR, aiming for higher-affinity mutants. The mutations were inserted downstream of the P_BAD promoter in the pBAD24 vector, creating a mutant library. This was then transformed into E. coli-competent cells. As a result, we obtained a Cs⁺-resistant strain, ZX-1, capable of thriving in 400 mM CsCl—a concentration too high for ordinary E. coli. Unlike the parent strain Mach1^™, which struggled in 300 mM CsCl, ZX-1 showed robust growth even in 700 mM CsCl. After 700 mM CsCl treatment, the 70S ribosome of Mach1^™ collapsed, whereas ZX-1 and its derivative ΔZX-1/pBR322ΔAp remained stable. This means that the ribosomes of ZX-1 are more stable to high Cs⁺. The inverted membrane vesicles from strain ZX-1 showed an apparent K_m value of 28.7 mM (pH 8.5) for Cs⁺/H⁺ antiport activity, indicating an approximately 12.9-fold increase in Cs⁺ affinity. Remarkably, the entire plasmid isolated from ZX-1, including the TS_cshA region, was mutation-free. Subsequent whole-genome analysis of ZX-1 identified multiple SNPs on the chromosome that differed from those in the parent strain. No mutations in transporter-related genes were identified in ZX-1. However, three mutations emerged as significant: genes encoding the ribosomal bS6 modification enzyme RimK, the phage lysis regulatory protein LysB, and the flagellar base component protein FlgG. These mutations are hypothesized to affect post-translational modifications, influencing the K_m value of TS_CshA and accessory protein expression. This study unveils a novel Cs⁺ resistance mechanism in ZX-1, enhancing our understanding of Cs⁺ resistance and paving the way for developing technology to recover radioactive Cs⁺ from water using TS_CshA-expressing inverted membrane vesicles.