AUTHOR=Hu Jing-rong , Li Jin-meng , Wang Hai-yan , Sun Mei-li , Huang Chun-yang , Wang Han-cheng 

TITLE=Analysis of growth dynamics in five different media and metabolic phenotypic characteristics of Piriformospora indica

JOURNAL=Frontiers in Microbiology

VOLUME=14

YEAR=2024

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1301743

DOI=10.3389/fmicb.2023.1301743

ISSN=1664-302X

ABSTRACT=<p><italic>Piriformospora indica</italic> is an important endophytic fungus with broad potential for alleviating biotic and abiotic stress on host plants. This study monitored the growth dynamics of <italic>P. indica</italic> on five commonly used artificial media for microorganisms and analyzed its metabolic characteristics using Biolog Phenotype Microarray (PM) technology. The results showed that <italic>P. indica</italic> grew fastest on Potato Dextrose Agar (PDA), followed by Kidney Bean Agar (KBA), Alkyl Ester Agar (AEA), Oatmeal Agar (OA), and Luria-Bertani Agar (LB), and the most suitable medium for spore production was OA. Using Biolog PM1-10, 950 metabolic phenotypes of <italic>P. indica</italic> were obtained. <italic>P. indica</italic> could metabolize 87.89% of the tested carbon sources, 87.63% of the tested nitrogen sources, 96.61% of the tested phosphorus sources, and 100% of the tested sulfur sources. <italic>P. indica</italic> displayed 92 kinds of tested biosynthetic pathways, and it could grow under 92 kinds of tested osmotic pressures and 88 kinds of tested pH conditions. PM plates 1-2 revealed 43 efficient carbon sources, including <italic>M</italic>-Hydroxyphenyl acid, <italic>N</italic>-Acetyl-<italic>D</italic>-Glucosamine, Tyramine, Maltotrios, α<italic>-D</italic>-Glucosine, <italic>I</italic>-Erythritol, <italic>L</italic>-Valine, <italic>D</italic>-Melezitose, <italic>D</italic>-Tagatose, and Turanose. PM plates 3,6-8 indicated 170 efficient nitrogen sources, including Adenosine, Inosine Allantoin, <italic>D, L</italic>-Lactamide, Arg-Met, lle-Trp, Ala-Arg, Thr-Arg, Trp-Tyr, Val-Asn, Gly-Gly-<italic>D</italic>-Leu, Gly-Gly-Phe, and Leu-Leu-Leu. This study demonstrates that <italic>P. indica</italic> can metabolize a variety of substrates, such as carbon and nitrogen sources, and has a wide range of environmental adaptability. The growth dynamics on artificial culture media and metabolic phenotypes of <italic>P. indica</italic> can be used to investigate its biological characteristics, screen for more suitable growth and sporulation conditions, and elucidate the physiological mechanisms that enhance the stress resistance of host plants. This study provides a theoretical basis for its better application in agriculture.</p>