AUTHOR=Qin Zhenyang , Peng Kun , Feng Yang , Wang Yilin , Huang Bowen , Tian Ziqi , Ouyang Ping , Huang Xiaoli , Chen Defang , Lai Weimin , Geng Yi 

TITLE=Transcriptome reveals the role of the htpG gene in mediating antibiotic resistance through cell envelope modulation in Vibrio mimicus SCCF01

JOURNAL=Frontiers in Microbiology

VOLUME=14

YEAR=2024

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1295065

DOI=10.3389/fmicb.2023.1295065

ISSN=1664-302X

ABSTRACT=<p>HtpG, a bacterial homolog of the eukaryotic 90 kDa heat-shock protein (Hsp90), represents the simplest member of the heat shock protein family. While the significance of Hsp90 in fungal and cancer drug resistance has been confirmed, the role of HtpG in bacterial antibiotic resistance remains largely unexplored. This research aims to investigate the impact of the <italic>htpG</italic> gene on antibiotic resistance in <italic>Vibrio mimicus</italic>. Through the creation of <italic>htpG</italic> gene deletion and complementation strains, we have uncovered the essential role of <italic>htpG</italic> in regulating the structural integrity of the bacterial cell envelope. Our transcriptomics analysis demonstrates that the deletion of <italic>htpG</italic> increases the sensitivity of <italic>V. mimicus</italic> to antimicrobial peptides, primarily due to upregulated lipopolysaccharide synthesis, reduced glycerophospholipid content, and weakened efflux pumps activity. Conversely, reduced sensitivity to β-lactam antibiotics in the Δ<italic>htpG</italic> strain results from decreased peptidoglycan synthesis and dysregulated peptidoglycan recycling and regulation. Further exploration of specific pathway components is essential for a comprehensive understanding of <italic>htpG</italic>-mediated resistance mechanisms, aiding in the development of antimicrobial agents. To our knowledge, this is the first effort to explore the relationship between <italic>htpG</italic> and drug resistance in bacteria.</p>