AUTHOR=Yang Han , Cui Shuning , Wei Yanli , Li Hongmei , Hu Jindong , Yang Kai , Wu Yuanzheng , Zhao Zhongjuan , Li Jishun , Wang Yilian , Yang Hetong 

TITLE=Antagonistic effects of Talaromyces muroii TM28 against Fusarium crown rot of wheat caused by Fusarium pseudograminearum

JOURNAL=Frontiers in Microbiology

VOLUME=14

YEAR=2024

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1292885

DOI=10.3389/fmicb.2023.1292885

ISSN=1664-302X

ABSTRACT=<p>Fusarium crown rot (FCR) caused by <italic>Fusarium pseudograminearum</italic> is a serious threat to wheat production worldwide. This study aimed to assess the effects of <italic>Talaromyces muroii</italic> strain TM28 isolated from root of <italic>Panax quinquefolius</italic> against <italic>F. pseudograminearum</italic>. The strain of TM28 inhibited mycelial growth of <italic>F. pseudograminearum</italic> by 87.8% at 72 h, its cell free fermentation filtrate had a strong antagonistic effect on mycelial growth and conidial germination of <italic>F. pseudograminearum</italic> by destroying the integrity of the cell membrane. In the greenhouse, TM28 significantly increased wheat fresh weight and height in the presence of pathogen <italic>Fp</italic>, it enhanced the antioxidant defense activity and ameliorated the negative effects of <italic>F. pseudograminearum</italic>, including disease severity and pathogen abundance in the rhizosphere soil, root and stem base of wheat. RNA-seq of <italic>F. pseudograminearum</italic> under TM28 antagonistic revealed 2,823 differentially expressed genes (DEGs). Most DEGs related to cell wall and cell membrane synthesis were significantly downregulated, the culture filtrate of TM28 affected the pathways of fatty acid synthesis, steroid synthesis, glycolysis, and the citrate acid cycle. <italic>T. muroii</italic> TM28 appears to have significant potential in controlling wheat Fusarium crown rot caused by <italic>F. pseudograminearum.</italic></p>