AUTHOR=Liu Songyi , Wang Ziyan , Wang Ze , Wu Qiaoli , Zhou Jun , Wang Rixin , Han Jiaojiao , Su Xiurong 

TITLE=Comparison of the gut microbiota and metabolism in different regions of Red Swamp Crayfish (Procambarus clarkii)

JOURNAL=Frontiers in Microbiology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1289634

DOI=10.3389/fmicb.2023.1289634

ISSN=1664-302X

ABSTRACT=<sec><title>Background</title><p>The gut microbiota is very important for maintaining the homeostasis and health of crustaceans. Many factors affect the gut microbiota of crustaceans, one of which is temperature. However, it is currently unclear how temperature affects the gut microbiota and metabolites of <italic>Procambarus clarkii</italic>.</p></sec><sec><title>Methods</title><p>Using metagenomic sequencing and gas chromatography–mass spectrometry (GC–MS) techniques, the gut microbiota and metabolites of <italic>P. clarkii</italic> from Hubei (HB), Jiangsu (JS), Shandong (SD), and Zhejiang (ZJ) in China were investigated.</p></sec><sec><title>Results</title><p>Under the impact of temperature, the gut microbiota and metabolites of <italic>P. clarkii</italic> exhibit a specific trend of change. The primary pathogenic bacteria affecting <italic>P. clarkii</italic> are <italic>Citrobacter, Enterobacterium</italic>, and <italic>Aeromonas</italic>, which are affected by temperature. Two metabolites, namely, sugars and amino acids, are regulated by temperature.</p></sec><sec><title>Implication</title><p>This study demonstrated that the gut microbiota and gut metabolites of <italic>P. clarkii</italic> were considerably affected by temperature. It provides a theoretical basis for the systematic study of <italic>P. clarkii</italic> and provides a basis for a healthy culture of <italic>P. clarkii</italic>.</p></sec>