AUTHOR=Lv Qiulan , Zhou Jun , Wang Changyao , Yang Xiaomin , Han Yafei , Zhou Quan , Yao Ruyong , Sui Aihua 

TITLE=A dynamics association study of gut barrier and microbiota in hyperuricemia

JOURNAL=Frontiers in Microbiology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1287468

DOI=10.3389/fmicb.2023.1287468

ISSN=1664-302X

ABSTRACT=<sec><title>Introduction</title><p>The intricate interplay between gut microbiota and hyperuricemia remains a subject of growing interest. However, existing studies only provided snapshots of the gut microbiome at single time points, the temporal dynamics of gut microbiota alterations during hyperuricemia progression and the intricate interplay between the gut barrier and microbiota remain underexplored. Our investigation revealed compelling insights into the dynamic changes in both gut microbiota and intestinal barrier function throughout the course of hyperuricemia.</p></sec><sec><title>Methods</title><p>The hyperuricemia mice (HY) were given intragastric administration of adenine and potassium oxalate. Gut microbiota was analyzed by 16S rRNA sequencing at 3, 7, 14, and 21 days after the start of the modeling process. Intestinal permeability as well as LPS, TNF-α, and IL-1β levels were measured at 3, 7, 14, and 21 days.</p></sec><sec><title>Results</title><p>We discovered that shifts in microbial community composition occur prior to the onset of hyperuricemia, key bacterial <italic>Bacteroidaceae</italic>, <italic>Bacteroides</italic>, and <italic>Blautia</italic> exhibited reduced levels, potentially fueling microbial dysbiosis as the disease progresses. During the course of hyperuricemia, the dynamic fluctuations in both uric acid levels and intestinal barrier function was accompanied with the depletion of key beneficial bacteria, including <italic>Prevotellaceae</italic>, <italic>Muribaculum</italic>, <italic>Parabacteroides</italic>, <italic>Akkermansia</italic>, and <italic>Bacteroides</italic>, and coincided with an increase in pathogenic bacteria such as <italic>Oscillibacter</italic> and <italic>Ruminiclostridium</italic>. This microbial community shift likely contributed to elevated lipopolysaccharide (LPS) and pro-inflammatory cytokine levels, ultimately promoting metabolic inflammation. The decline of <italic>Burkholderiaceae</italic> and <italic>Parasutterella</italic> was inversely related to uric acid levels, Conversely, key families <italic>Ruminococcaceae</italic>, <italic>Family_XIII</italic>, genera <italic>Anaeroplasma</italic> exhibited positive correlations with uric acid levels. <italic>Akkermansiaceae</italic> and <italic>Bacteroidaceae</italic> demonstrating negative correlations, while LPS-containing microbiota such as <italic>Desulfovibrio</italic> and <italic>Enterorhabdus</italic> exhibited positive correlations with intestinal permeability.</p></sec><sec><title>Conclusion</title><p>In summary, this study offers a dynamic perspective on the complex interplay between gut microbiota, uric acid levels, and intestinal barrier function during hyperuricemia progression. Our study suggested that Ruminiclostridium, Bacteroides, Akkermansiaceae, Bilophila, Burkholderiaceae and Parasutterella were the key bacteria that play vital rols in the progress of hyperuricemia and compromised intestinal barrier, which provide a potential avenue for therapeutic interventions in hyperuricemia.</p></sec>