AUTHOR=Han Lianquan , Zhang Maolu , Li Fuwei , Su Jing , Wang Ruiming , Li Guiming , Yang Xiaohui 

TITLE=10-hydroxy-2-decenoic acid alleviates lipopolysaccharide-induced intestinal mucosal injury through anti-inflammatory, antioxidant, and gut microbiota modulation activities in chickens

JOURNAL=Frontiers in Microbiology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1285299

DOI=10.3389/fmicb.2023.1285299

ISSN=1664-302X

ABSTRACT=<sec><title>Introduction</title><p>This study aimed to investigated the effects of 10-hydroxy-2-decenoic acid (10-HDA) on the growth performance, intestinal barrier, inflammatory response, oxidative stress, and gut microbiota of chickens challenged with lipopolysaccharide (LPS).</p></sec><sec><title>Methods</title><p>A total of 240 one-day-old chickens were randomly assigned to five treatment groups: (1) control group (basal diet + saline); (2) LPS group (basal diet + LPS); (3) Chlortetracycline (CTC) group (basal diet containing 75 mg/kg CTC + LPS); (4) 0.1% 10-HDA group (basal diet containing 1 g/kg 10-HDA + LPS); and (5) 0.5% 10-HDA group (basal diet containing 5 g/kg 10-HDA + LPS). All chickens were injected intraperitoneally with 0.5 mg/kg body weight of either LPS or saline at 17, 19, and 21 days of age.</p></sec><sec><title>Results</title><p>The results showed that dietary 10-HDA supplementation attenuated the loss in growth performance caused by the LPS challenge (<italic>p</italic> &lt; 0.05). 10-HDA effectively alleviated LPS-induced intestinal mucosal injury, as evidenced by reduced bleeding, decreased serum diamine oxidase levels (<italic>p</italic> &lt; 0.05), and increased villus/crypt ratios of the jejunum and ileum (<italic>p</italic> &lt; 0.05). Dietary treatment with 0.1% 10-HDA reduced the concentrations of inflammatory cytokines (TNF-α, IL-1β, IL-6; <italic>p</italic> &lt; 0.05), and increased immunoglobulin (IgA, IgG) and antioxidant enzyme levels (CAT, GSH-px, T-SOD) in the serum of LPS-challenged chickens (<italic>p</italic> &lt; 0.05). These effects were similar to those observed in the CTC group. Moreover, 0.1% 10-HDA treatment reversed the LPS-induced variations in the mRNA expression of genes related to inflammation, antioxidant capacity, and intestinal tight junctions (<italic>p</italic> &lt; 0.05). 16S rRNA analysis revealed that 10-HDA supplementation increased the relative abundance of <italic>Faecalibacterium</italic> and <italic>Clostridia_UCG-014</italic> (<italic>p</italic> &lt; 0.05). Additionally, it decreased the abundance of <italic>Clostridia_vadinBB60_group</italic>, <italic>Eubacterium_nodatum_group</italic>, and <italic>UC5-1-2E3</italic> (<italic>p</italic> &lt; 0.05). These changes were correlated with reduced inflammation and improved antioxidant capacity in the LPS-challenged chickens.</p></sec><sec><title>Conclusion</title><p>Collectively, dietary 10-HDA supplementation alleviated LPS-induced intestinal mucosal injury and the loss of growth performance through anti-inflammatory, antioxidant, and gut microbiota modulation activities in chickens. Moreover, 0.1% 10-HDA supplementation had comparable or even better protection for LPS-challenged chickens than supplementation with antibiotics or 0.5% 10-HDA. 10-HDA has the potential to be used as an alternative to antibiotics in protecting the intestinal health and improving the performance of poultry.</p></sec>