AUTHOR=Shi Huineng , Yang Jianli , Li Qi , PinChu Cier , Song Zhanhua , Yang Honglei , Luo Yu , Liu Chunlan , Fan Wei 

TITLE=Diversity and correlation analysis of different root exudates on the regulation of microbial structure and function in soil planted with Panax notoginseng

JOURNAL=Frontiers in Microbiology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1282689

DOI=10.3389/fmicb.2023.1282689

ISSN=1664-302X

ABSTRACT=<sec><title>Introduction</title><p>Specific interactions between root exudates and soil microorganisms has been proposed as one of the reasons accounting for the continuous cropping obstacle (CCO) of <italic>Panax notoginseng</italic>. However, rotation of other crops on soils planted with <italic>P. notoginseng</italic> (SPP) did not show CCO, suggesting that root exudates of different crops differentially regulate soil microorganisms in SPP.</p></sec><sec><title>Methods</title><p>Here, we investigated the microbial community structure and specific interaction mechanisms of the root exudates of the four plant species, <italic>P. notoginseng</italic> (Pn), <italic>Zea mays</italic> (Zm), <italic>Nicotiana tabacum</italic> (Nt) and <italic>Perilla frutescens</italic> (Pf), in SPP by static soil culture experiment.</p></sec><sec><title>Results</title><p>The results showed that the chemical diversity of root exudates varied significantly among the four plant species. Pn had the highest number of unique root exudates, followed by Nt, Zm and Pf. Terpenoids, flavonoids, alkaloids and phenolic acids were the most abundant differentially accumulated metabolites (DAMs) in Pn, Nt, Zm and Pf, respectively. However, lipids were the most abundant common DAMs among Zm Nt and Pf. Pn root exudates decreased the relative abundance of bacteria, but increased that of fungi. While specific DAMs in Pn enriched <italic>Phenylobacterium_zucineum</italic>, <italic>Sphingobium_yanoikuyae</italic>, <italic>Ophiostoma_ulmi</italic> and functional pathways of Nucleotide excision repair, Streptomycin biosynthesis, Cell cycle-Caulobacter and Glycolysis/Gluconeogenesis, it inhibited <italic>Paraburkholderia _caledonica</italic> and <italic>Ralstonia_pickettii</italic>. However, common DAMs in Zm, Nt and Pf had opposite effects. Moreover, common DAMs in Zm, Nt and Pf enriched <italic>Ralstonia_pseudosolanacearum</italic> and functional pathway of Xylene degradation; unique DAMs in Zm enriched <italic>Talaromyces_purcureogeneus</italic>, while inhibiting <italic>Fusarium_tricinctum</italic> and functional pathways of Nucleotide excision repair and Alanine, aspartate and glutamate metabolism; unique DAMs in Pf enriched <italic>Synchytrium_taraxaci</italic>.</p></sec><sec><title>Discussion</title><p>The core strains identified that interact with different root exudates will provide key clues for regulation of soil microorganisms in <italic>P. notoginseng</italic> cultivation to alleviate CCO.</p></sec>