AUTHOR=Sun Yunhao , Su Yutong , Meng Zhen , Zhang Jie , Zheng Li , Miao Shuang , Qin Di , Ruan Yulan , Wu Yanhui , Xiong Lina , Yan Xun , Dong Zhangyong , Cheng Ping , Shao Mingwei , Yu Guohui 

TITLE=Biocontrol of bacterial wilt disease in tomato using Bacillus subtilis strain R31

JOURNAL=Frontiers in Microbiology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1281381

DOI=10.3389/fmicb.2023.1281381

ISSN=1664-302X

ABSTRACT=<p>Bacterial wilt disease caused by <italic>Ralstonia solanacearum</italic> is a widespread, severe plant disease. Tomato (<italic>Solanum lycopersicum</italic>), one of the most important vegetable crops worldwide, is particularly susceptible to this disease. Biological control offers numerous advantages, making it a highly favorable approach for managing bacterial wilt. In this study, the results demonstrate that treatment with the biological control strain <italic>Bacillus subtilis</italic> R31 significantly reduced the incidence of tomato bacterial wilt. In addition, R31 directly inhibits the growth of <italic>R. solanacearum</italic>, and lipopeptides play an important role in this effect. The results also show that R31 can stably colonize the rhizosphere soil and root tissues of tomato plants for a long time, reduce the <italic>R. solanacearum</italic> population in the rhizosphere soil, and alter the microbial community that interacts with <italic>R. solanacearum</italic>. This study provides an important theoretical basis for elucidating the mechanism of <italic>B. subtilis</italic> as a biological control agent against bacterial wilt and lays the foundation for the optimization and promotion of other agents such as R31.</p>