AUTHOR=Qiu Binxu , Shen Zixiong , Yang Dongliang , Qin Xinxin , Ren Wenyong , Wang Quan TITLE=Gut microbiota and common gastrointestinal diseases: a bidirectional two-sample Mendelian randomized study JOURNAL=Frontiers in Microbiology VOLUME=14 YEAR=2023 URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1273269 DOI=10.3389/fmicb.2023.1273269 ISSN=1664-302X ABSTRACT=Background

Several recent studies have shown an association between gut microbiota and gastrointestinal diseases. However, the causal relationship between gut microbiota and gastrointestinal disorders is unclear.

Methods

We assessed causal relationships between gut microbiota and eight common gastrointestinal diseases using Mendelian randomization (MR) analyses. IVW results were considered primary results. Cochrane’s Q and MR-Egger tests were used to test for heterogeneity and pleiotropy. Leave-one-out was used to test the stability of the MR results, and Bonferroni correction was used to test the strength of the causal relationship between exposure and outcome.

Results

MR analyses of 196 gut microbiota and eight common gastrointestinal disease phenotypes showed 62 flora and common gastrointestinal diseases with potential causal relationships. Among these potential causal relationships, after the Bonferroni-corrected test, significant causal relationships remained between Genus Oxalobacter and CD (OR = 1.29, 95% CI: 1.13–1.48, p = 2.5 × 10–4, q = 4.20 × 10–4), and between Family Clostridiaceae1 and IBS (OR = 0.9967, 95% CI: 0.9944–0.9991, p = 1.3 × 10–3, q = 1.56 × 10–3). Cochrane’s Q-test showed no significant heterogeneity among the various single nucleotide polymorphisms (SNPs). In addition, no significant level of pleiotropy was found according to the MR-Egger.

Conclusion

This study provides new insights into the mechanisms of gut microbiota-mediated gastrointestinal disorders and some guidance for targeting specific gut microbiota for treating gastrointestinal disorders.