AUTHOR=Tan Sheng , Zhang Jie , Peng Yonglin , Du Wenfei , Yan Jingxuan , Fang Qin 

TITLE=Integrative transcriptome analysis reveals alternative polyadenylation potentially contributes to GCRV early infection

JOURNAL=Frontiers in Microbiology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1269164

DOI=10.3389/fmicb.2023.1269164

ISSN=1664-302X

ABSTRACT=<sec><title>Introduction</title><p>Grass carp reovirus (GCRV), a member of the <italic>Aquareovirus genus</italic> in the <italic>Reoviridae</italic> family, is considered to be the most pathogenic aquareovirus. Productive viral infection requires extensive interactions between viruses and host cells. However, the molecular mechanisms underlying GCRV early infection remains elusive.</p></sec><sec><title>Methods</title><p>In this study we performed transcriptome and DNA methylome analyses with <italic>Ctenopharyngodon idellus</italic> kidney (CIK) cells infected with GCRV at 0, 4, and 8 h post infection (hpi), respectively.</p></sec><sec><title>Results</title><p>We found that at early infection stage the differentially expressed genes related to defense response and immune response in CIK cells are activated. Although DNA methylation pattern of CIK cells 8 hpi is similar to mock-infected cells, we identified a considerable number of genes that selectively utilize alternative polyadenylation sites. Particularly, we found that biological processes of cytoskeleton organization and regulation of microtubule polymerization are statistically enriched in the genes with altered 3’UTRs.</p></sec><sec><title>Discussion</title><p>Our results suggest that alternative polyadenylation potentially contributes to GCRV early infection.</p></sec>