AUTHOR=Jiang Zhu-Ming , Mou Tong , Sun Ye , Su Jing , Yu Li-Yan , Zhang Yu-Qin 

TITLE=Environmental distribution and genomic characteristics of Solirubrobacter, with proposal of two novel species

JOURNAL=Frontiers in Microbiology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1267771

DOI=10.3389/fmicb.2023.1267771

ISSN=1664-302X

ABSTRACT=<p><italic>Solirubrobacter</italic> spp. were abundant in soil samples collected from deserts and other areas with high UV radiation. In addition, a novel <italic>Solirubrobacter</italic> species, with strain CPCC 204708<italic><sup>T</sup></italic> as the type, was isolated and identified from sandy soil sample collected from the Badain Jaran Desert of the Inner Mongolia autonomous region. Strain CPCC 204708<italic><sup>T</sup></italic> was Gram-stain positive, rod-shaped, non-motile, non-spore-forming, and grew optimally at 28–30°C, pH 7.0–8.0, and in the absence of NaCl. Analysis of the 16S rRNA gene sequence of strain CPCC 204708<italic><sup>T</sup></italic> showed its identity within the genus <italic>Solirubrobacter</italic>, with highest nucleotide similarities (97.4–98.2%) to other named <italic>Solirubrobacter</italic> species. Phylogenetic and genomic analyses indicated that the strain was most closely related to <italic>Solirubrobacter phytolaccae</italic> KCTC 29190<italic><sup>T</sup></italic>, while represented a distinct species, as confirmed from physiological properties and comparison. The name <italic>Solirubrobacter deserti</italic> sp. nov. was consequently proposed, with CPCC 204708<italic><sup>T</sup></italic> (= DSM 105495<italic><sup>T</sup></italic> = NBRC 112942<italic><sup>T</sup></italic>) as the type strain. Genomic analyses of the <italic>Solirubrobacter</italic> spp. also suggested that <italic>Solirubrobacter</italic> sp. URHD0082 represents a novel species, for which the name <italic>Candidatus</italic> “Solirubrobacter pratensis” sp. nov. was proposed. Genomic analysis of CPCC 204708<italic><sup>T</sup></italic> revealed the presence of genes related to its adaptation to the harsh environments of deserts and may also harbor genes functional in plant-microbe interactions. Pan-genomic analysis of available <italic>Solirubrobacter</italic> spp. confirmed the presence of many of the above genes as core components of <italic>Solirubrobacter</italic> genomes and suggests they may possess beneficial potential for their associate plant and may be important resources for bioactive compounds.</p>