AUTHOR=Li Xiao Li , Lv Xin Yang , Ji Jun Bin , Wang Wei Duo , Wang Ji , Wang Cong , He Hai Bin , Ben Ai Ling , Liu Ting Li 

TITLE=Complete genome sequence of Nguyenibacter sp. L1, a phosphate solubilizing bacterium isolated from Lespedeza bicolor rhizosphere

JOURNAL=Frontiers in Microbiology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1257442

DOI=10.3389/fmicb.2023.1257442

ISSN=1664-302X

ABSTRACT=<p>Phosphorus (P) deficiency is a predominant constraint on plant growth in acidified soils, largely due to the sequestration of P by toxic aluminum (Al) compounds. Indigenous phosphorus-solubilizing bacteria (PSBs) capable of mobilizing Al-P in these soils hold significant promise. A novel Al-P-solubilizing strain, Al-P <italic>Nguyenibacter</italic> sp. L1, was isolated from the rhizosphere soil of healthy <italic>Lespedeza bicolor</italic> plants indigenous to acidic terrains. However, our understanding of the genomic landscape of bacterial species within the genus <italic>Nguyenibacter</italic> remains in its infancy. To further explore its biotechnological potentialities, we sequenced the complete genome of this strain, employing an amalgamation of Oxford Nanopore ONT and Illumina sequencing platforms. The resultant genomic sequence of <italic>Nguyenibacter</italic> sp. L1 manifests as a singular, circular chromosome encompassing 4,294,433 nucleotides and displaying a GC content of 66.73%. The genome was found to host 3,820 protein-coding sequences, 12 rRNAs, and 55 tRNAs. Intriguingly, annotations derived from the eggNOG and KEGG databases indicate the presence of genes affiliated with phosphorus solubilization and nitrogen fixation, including <italic>isc</italic>U, <italic>gln</italic>A, and <italic>glt</italic>B/D associated with nitrogen fixation, and <italic>pqq</italic>BC associated with inorganic phosphate dissolution. Several bioactive secondary metabolite genes in the genome, including <italic>pqq</italic>CDE, phytoene synthase and squalene synthase predicted by antiSMASH. Moreover, we uncovered a complete metabolic pathway for ammonia, suggesting an ammonia-affinity property inherent to <italic>Nguyenibacter</italic> sp. L1. This study verifies the nitrogen-fixing and phosphate-dissolving abilities of <italic>Nguyenibacter</italic> sp. L1 at the molecular level through genetic screening and analysis. The insights gleaned from this study offer strategic guidance for future strain enhancement and establish a strong foundation for the potential incorporation of this bacterium into agricultural practices.</p>