AUTHOR=Leñini Cecilia , Rodriguez Ayala Facundo , Goñi Anibal Juan , Rateni Liliana , Nakamura Akira , Grau Roberto Ricardo 

TITLE=Probiotic properties of Bacillus subtilis DG101 isolated from the traditional Japanese fermented food nattō

JOURNAL=Frontiers in Microbiology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1253480

DOI=10.3389/fmicb.2023.1253480

ISSN=1664-302X

ABSTRACT=<p>Spore-forming probiotic bacteria offer interesting properties as they have an intrinsic high stability, and when consumed, they are able to survive the adverse conditions encountered during the transit thorough the host gastrointestinal (GI) tract. A traditional healthy food, nattō, exists in Japan consisting of soy fermented by the spore-forming bacterium <italic>Bacillus subtilis</italic> natto. The consumption of nattō is linked to many beneficial health effects, including the prevention of high blood pressure, osteoporosis, and cardiovascular-associated disease. We hypothesize that the bacterium <italic>B. subtilis</italic> natto plays a key role in the beneficial effects of nattō for humans. Here, we present the isolation of <italic>B. subtilis</italic> DG101 from nattō and its characterization as a novel spore-forming probiotic strain for human consumption. <italic>B. subtilis</italic> DG101 was non-hemolytic and showed high tolerance to lysozyme, low pH, bile salts, and a strong adherence ability to extracellular matrix proteins (i.e., fibronectin and collagen), demonstrating its potential application for competitive exclusion of pathogens. <italic>B. subtilis</italic> DG101 forms robust liquid and solid biofilms and expresses several extracellular enzymes with activity against food diet-associated macromolecules (i.e., proteins, lipids, and polysaccharides) that would be important to improve food diet digestion by the host. <italic>B. subtilis</italic> DG101 was able to grow in the presence of toxic metals (i.e., chromium, cadmium, and arsenic) and decreased their bioavailability, a feature that points to this probiotic as an interesting agent for bioremediation in cases of food and water poisoning with metals. In addition, <italic>B. subtilis</italic> DG101 was sensitive to antibiotics commonly used to treat infections in medical settings, and at the same time, it showed a potent antimicrobial effect against pathogenic bacteria and fungi. In mammalians (i.e., rats), <italic>B. subtilis</italic> DG101 colonized the GI tract, and improved the lipid and protein serum homeostasis of animals fed on the base of a normal- or a deficient-diet regime (dietary restriction). In the animal model for longevity studies, <italic>Caenorhabditis elegans</italic>, <italic>B. subtilis</italic> DG101 significantly increased the animal lifespan and prevented its age-related behavioral decay. Overall, these results demonstrate that <italic>B. subtilis</italic> DG101 is the key component of nattō with interesting probiotic properties to improve and protect human health.</p>