AUTHOR=Yu Xiaoqin , Müller Werner E. G. , Frank Marian , Gao Ying , Guo Zhiyong , Zou Kun , Proksch Peter , Liu Zhen 

TITLE=Caryophyllene-type sesquiterpenes from the endophytic fungus Pestalotiopsis lespedezae through an OSMAC approach

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 14 - 2023

YEAR=2024

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1248896

DOI=10.3389/fmicb.2023.1248896

ISSN=1664-302X

ABSTRACT=Two new caryophyllene-type sesquiterpenes pestalotiopsins U and V (1 and 2) and three known compounds pestalotiopsin B (7), pestaloporinate B (8) and pestalotiopsin C (9) were isolated by cultivation of the endophytic fungus Pestalotiopsis lespedezae on solid rice medium while four additional new caryophyllenes pestalotiopsins W-Z (3-6) were obtained when 3.5% NaI was added to fungal culture medium. The structures of the new compounds were determined by HRESIMS and 1D/2D nuclear magnetic resonance data. Compounds 1-9 were tested for cytotoxicity against the mouse lymphoma cell line L5178Y, but only 6 displayed significant activity with an IC50 value of 2.4 μM.