AUTHOR=Yu Xiaoqin , Müller Werner E. G. , Frank Marian , Gao Ying , Guo Zhiyong , Zou Kun , Proksch Peter , Liu Zhen 

TITLE=Caryophyllene-type sesquiterpenes from the endophytic fungus Pestalotiopsis lespedezae through an OSMAC approach

JOURNAL=Frontiers in Microbiology

VOLUME=14

YEAR=2024

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1248896

DOI=10.3389/fmicb.2023.1248896

ISSN=1664-302X

ABSTRACT=<p>Two new caryophyllene-type sesquiterpenes pestalotiopsins U and V (<bold>1</bold> and <bold>2</bold>) and three known compounds pestalotiopsin B (<bold>7</bold>), pestaloporinate B (<bold>8</bold>), and pestalotiopsin C (<bold>9</bold>) were isolated by the cultivation of the endophytic fungus <italic>Pestalotiopsis lespedezae</italic> on solid rice medium, while four additional new caryophyllene pestalotiopsins W–Z (<bold>3</bold>–<bold>6</bold>) were obtained when 3.5% NaI was added to the fungal culture medium. The structures of the new compounds were determined by HRESIMS and 1D/2D nuclear magnetic resonance data. Compounds <bold>1</bold>–<bold>9</bold> were tested for cytotoxicity against the mouse lymphoma cell line L5178Y, but only <bold>6</bold> displayed significant activity with an IC<sub>50</sub> value of 2.4 μM.</p>