AUTHOR=Fernández-García Laura , Tomás María , Wood Thomas K. 

TITLE=Ribosome inactivation by Escherichia coli GTPase RsgA inhibits T4 phage

JOURNAL=Frontiers in Microbiology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1242163

DOI=10.3389/fmicb.2023.1242163

ISSN=1664-302X

ABSTRACT=<sec><title>Introduction</title><p>Bacteria must combat phages, and myriad bacterial anti-phage systems have been discovered that reduce host metabolism, for example, by depleting energetic compounds like ATP and NAD<sup>+</sup>. Hence, these systems indirectly inhibit protein production. Surprisingly, direct reduction of ribosome activity has not been demonstrated to thwart phage.</p></sec><sec><title>Methods</title><p>Here, by producing each of the 4,287 <italic>Escherichia coli</italic> proteins and selecting for anti-phage activity that leads to enhanced growth, we investigated the role of host proteins in phage inhibition.</p></sec><sec><title>Results and discussion</title><p>We identified that <italic>E. coli</italic> GTPase RsgA inhibits lytic phage T4 by inactivating ribosomes.</p></sec>