AUTHOR=Guo Huiyan , Xu Chuantao , Wang Fei , Jiang Lianqiang , Lei Xiao , Zhang Mingjin , Li Rui , Lan Xinyu , Xia Zihao , Wang Zhiping , Wu Yuanhua 

TITLE=Transcriptome sequencing and functional verification revealed the roles of exogenous magnesium in tobacco anti-PVY infection

JOURNAL=Frontiers in Microbiology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1232279

DOI=10.3389/fmicb.2023.1232279

ISSN=1664-302X

ABSTRACT=<p>Potato virus Y (PVY) infection causes necrosis and curling of leaves, which seriously affect the yield and quality of Solanaceous crops. The roles of nutrient elements in the regulation of plant resistance to virus infection has been widely reported, while the mechanisms are poorly studied. Previous studies in our laboratory have demonstrated that foliar spraying of MgSO<sub>4</sub> could induce <italic>Nicotiana tabacum</italic> resistance to PVY by increasing the activity of defense-related enzymes. Consistent with the results, we found that exogenous magnesium (Mg) had a certain effect on <italic>N. tabacum</italic> anti-PVY infection. Meanwhile, Illumina RNA sequencing revealed that Mg induced resistance to PVY infection was mainly by regulating carbohydrate metabolism and transportation, nitrogen metabolism, Ca<sup>2+</sup> signal transduction and oxidative phosphorylation. Moreover, we used virus-induced gene silencing assays to verify the function of homologs of five <italic>N. tabacum</italic> genes involved in above pathways in <italic>N. benthamiana</italic>. The results showed that <italic>NbTPS</italic> and <italic>NbGBE</italic> were conducive to PVY infection, while <italic>NbPPases</italic> and <italic>NbNR</italic> were related to resistance to PVY infection. These results suggested a novel strategy for resistance to PVY infection and provided a theoretical basis for virus-resistance breeding.</p>