AUTHOR=Wang Xiaogang , Zheng Yaojun , Chen Xiang , Peng Chen , Zhou Shizhen , Shen Sunan , Zhao Shuli , Wang Tingting 

TITLE=2bRAD-M reveals the difference in microbial distribution between cancerous and benign ovarian tissues

JOURNAL=Frontiers in Microbiology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1231354

DOI=10.3389/fmicb.2023.1231354

ISSN=1664-302X

ABSTRACT=<p>The development of ovarian cancer is closely related to various factors, such as environmental, genetic and microbiological factors. In previous research, bacteria were identified in human tumors by 16S rRNA sequencing. However, the microbial biomass in tumor tissue is too low and cannot be accurately identified by 16S rRNA sequencing. In our study, we employ 2bRAD sequencing for Microbiome (2bRAD-M), a new sequencing technology capable of accurately characterizing the low biomass microbiome (bacteria, fungi and archaea) at species resolution. Here we surveyed 20 ovarian samples, including 10 ovarian cancer samples and 10 benign ovarian samples. The sequencing results showed that a total of 373 microbial species were identified in both two groups, of which 90 species shared in the two groups. The Meta statistic indicated that <italic>Chlamydophila_abortus</italic> and <italic>CAG-873_sp900550395</italic> were increased in the ovarian cancer tissues, while <italic>Lawsonella_clevelandensis_A</italic>, <italic>Ralstonia_sp001078575</italic>, <italic>Brevundimonas_aurantiaca</italic>, <italic>Ralstonia_sp900115545</italic>, <italic>Ralstonia_pickettii</italic>, <italic>Corynebacterium_kefirresidentii</italic>, <italic>Corynebacterium_sp000478175</italic>, <italic>Brevibacillus_D_fluminis</italic>, <italic>Ralstonia_sp000620465</italic>, and <italic>Ralstonia_mannitolilytica</italic> were more abundant in the benign ovarian tissues. This is the first use of 2bRAD-M technique to provide an important hint for better understanding of the ovarian cancer microbiome.</p>