AUTHOR=Vion Charlotte , Brambati Mathilde , Da Costa Grégory , Richard Tristan , Marullo Philippe 

TITLE=Endo metabolomic profiling of flor and wine yeasts reveals a positive correlation between intracellular metabolite load and the specific glycolytic flux during wine fermentation

JOURNAL=Frontiers in Microbiology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1227520

DOI=10.3389/fmicb.2023.1227520

ISSN=1664-302X

ABSTRACT=<p>This study explored the intracellular metabolic variations between 17 strains of <italic>Saccharomyces cerevisiae</italic> belonging to two different genetic populations: <italic>flor</italic> and wine yeasts, in the context of alcoholic fermentation. These two populations are closely related as they share the same ecological niche but display distinct genetic characteristics. A protocol was developed for intracellular metabolites extraction and <sup>1</sup>H-NMR analysis. This methodology allowed us to identify and quantify 21 intracellular metabolites at two different fermentation steps: the exponential and stationary phases. This work provided evidence of significant differences in the abundance of intracellular metabolites, which are strain- and time-dependent, thus revealing complex interactions. Moreover, the differences in abundance appeared to be correlated with life-history traits such as average cell size and specific glycolytic flux, which revealed unsuspected phenotypic correlations between metabolite load and fermentation activity.</p>