AUTHOR=Ngamcharungchit Chananan , Matsumoto Atsuko , Suriyachadkun Chanwit , Panbangred Watanalai , Inahashi Yuki , Intra Bungonsiri 

TITLE=Nonomuraea corallina sp. nov., isolated from coastal sediment in Samila Beach, Thailand: insights into secondary metabolite synthesis as anticancer potential

JOURNAL=Frontiers in Microbiology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1226945

DOI=10.3389/fmicb.2023.1226945

ISSN=1664-302X

ABSTRACT=<p>A novel marine actinomycete, designated strain MCN248<sup>T</sup>, was isolated from the coastal sediment in Songkhla Province, Thailand. Based on the 16S rRNA gene sequences, the new isolate was closely related to <italic>Nonomuraea harbinensis</italic> DSM45887<sup>T</sup> (99.2%) and <italic>Nonomuraea ferruginea</italic> DSM43553<sup>T</sup> (98.6%). Phylogenetic analyzes based on the 16S rRNA gene sequences showed that strain MCN248<sup>T</sup> was clustered with <italic>Nonomuraea harbinensis</italic> DSM45887<sup>T</sup> and <italic>Nonomuraea ferruginea</italic> DSM43553<sup>T</sup>. However, the digital DNA–DNA hybridization analyzes presented a low relatedness of 40.2% between strain MCN248<sup>T</sup> and the above closely related strains. This strain contained meso-diaminopimelic acid. The acyl type of the peptidoglycan was acetyl, and mycolic acids were absent. The major menaquinones were MK-9(H<sub>2</sub>) and MK-9(H<sub>4</sub>). The whole cell sugars consisted of madurose, ribose, mannose, and glucose. Diphosphatidylglycerol, hydroxyl-phosphatidylethanolamine, phosphatidylethanolamine, phosphatidylinositol, and phosphatidylglycerol were detected as the major phospholipids. The predominant cellular fatty acids were <italic>iso</italic>-C<sub>16:0</sub> (40.4%), 10-methyl-C<sub>17:0</sub> (22.1%), and C<sub>17:1</sub><inline-formula><mml:math id="M1" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mi>ω</mml:mi></mml:math></inline-formula>8c (10.9%). The DNA G + C content of the genomic DNA was 71.7%. With <italic>in silico</italic> analyzes, the antiSMASH platform uncovered a diverse 29 secondary metabolite biosynthesis arsenal, including non-ribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) of strain MCN248<sup>T</sup>, with a high prevalence of gene cluster encoding pathways for the production of anticancer and cytotoxic compounds. Consistently, the crude extract could inhibit colorectal HCT-116 cancer cells at a final concentration of 50 μg/mL. Based on the polyphasic approach, strain MCN248 was designated as a novel species of the genus <italic>Nonomuraea</italic>, for which the name <italic>Nonomuraea corallina</italic> sp. nov. is proposed. The type strain of the type species is MCN248<sup>T</sup> (=NBRC115966<sup>T</sup> = TBRC17110<sup>T</sup>).</p>