AUTHOR=Roig Molina Francisco Jose , Amaro González Carmen , Alcaine Otín Alejandro , Carro Fernández Jesús 

TITLE=Vibrio vulnificus mutation rate: an in vitro approach

JOURNAL=Frontiers in Microbiology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1223293

DOI=10.3389/fmicb.2023.1223293

ISSN=1664-302X

ABSTRACT=<p><italic>Vibrio vulnificus</italic> is a multi-host pathogenic species currently subdivided into five phylogenetic lineages (L) plus one pathovar with the ability to infect fish due to a transmissible virulence plasmid. This plasmid (or a fragment of it) has been transmitted between lineages within the species, contributing to the evolution of <italic>V. vulnificus</italic>. This study aimed to provide an experimental approximation to the <italic>V. vulnificus</italic> mutation rate by determining spontaneous mutation rates from bacterial cultures of representants of the different lineages by whole-genome sequencing. To this purpose, synonymous SNP differences, i.e., spontaneous mutation not subjected to the evolutive forces, between initial and final culture after serial growth were evaluated and used for mutation rate calculation.</p>