AUTHOR=Liu Wenbin , Ou Peiyu , Tian Fangyuan , Liao Jingyang , Ma Yan , Wang Jie , Jin Xiaobao 

TITLE=Anti-Vibrio parahaemolyticus compounds from Streptomyces parvus based on Pan-genome and subtractive proteomics

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 14 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1218176

DOI=10.3389/fmicb.2023.1218176

ISSN=1664-302X

ABSTRACT=Vibrio parahaemolyticus is a foodborne pathogen commonly found in seafood, and drug resistance poses significant challenges to its control. This study aimed to identify novel drug targets for antibacterial drug discovery. Using pan-genome and subtractive proteomics, 22 potential antimicrobial drug targets were obtained from 58 V. parahaemolyticus genomes. After physiochemical checks, Flagellar motor switch protein FliN was selected as a therapeutic target. FliN was modeled and docked with Streptomyces parvus source compounds, and Actinomycin D was identified as a potential anti-V. parahaemolyticus agent with a strong binding energy. Experimental verification confirmed its effectiveness in killing V. parahaemolyticus and significantly inhibiting biofilm formation and motility. This study is the first to use pan-genome and subtractive proteomics to identify new antimicrobial targets for V. parahaemolyticus and to identify the anti-V. parahaemolyticus effect of Actinomycin D. These findings suggest potential avenues for the development of new antibacterial drugs to control V. parahaemolyticus infections.