AUTHOR=Xu Ailing , Liu Congcong , Zhao Shuke , Song Zhiwen , Sun Hui 

TITLE=Dynamic distribution of Massilia spp. in sewage, substrate, plant rhizosphere/phyllosphere and air of constructed wetland ecosystem

JOURNAL=Frontiers in Microbiology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1211649

DOI=10.3389/fmicb.2023.1211649

ISSN=1664-302X

ABSTRACT=<sec><title>Introduction</title><p><italic>Massilia</italic> bacteria are widely distributed and have various ecological functions. Preliminary studies have shown that <italic>Massilia</italic> is the dominant species in constructed wetland ecosystems, but its species composition and distribution in constructed wetlands are still unclear.</p></sec><sec><title>Methods</title><p>In this paper, the in-house-designed primers were used to construct a 16S rDNA clone library of <italic>Massilia</italic>. The RFLP sequence analysis method was used to analyze the diversity of <italic>Massilia</italic> clone library and the composition of <italic>Massilia</italic> in sewage, substrate, plant rhizosphere, plant phyllosphere and air in a constructed wetland sewage treatment system. Redundancy analysis (RDA) and canonical correspondence analysis (CCA) were used to analyze the correlation between environmental factors and the population characteristics of <italic>Massilia</italic> in the corresponding environment. The dominant species of <italic>Massilia</italic> were analyzed for differences.</p></sec><sec><title>Results</title><p>The results showed that the 16S rDNA clone library in primer 5 worked well. According to the clone library diversity index analysis, the richness of <italic>Massilia</italic> varied significantly in different environments in different seasons, where the overall summer and autumn richness was higher than that in the spring and winter. The relative abundance of 5 <italic>Massilia</italic> in the constructed wetland ecosystem was greater than 1% in all samples, which were <italic>M. alkalitolerans</italic>, <italic>M. albidiflava</italic>, <italic>M. aurea</italic>, <italic>M. brevitalea</italic>, and <italic>M. timonae</italic>. The seasonal variation of dominant genera was significantly correlated with environmental factors in constructed wetlands.</p></sec><sec><title>Discussion</title><p>The above results indicated that the species of <italic>Massilia</italic> were abundant and widely distributed in the constructed wetland ecosystem, and there were significant seasonal differences. In addition, the <italic>Massilia</italic> clone library of constructed wetland was constructed for the first time in this study and the valuable data of <italic>Massilia</italic> community structure were provided, which was conducive to the further study of microbial community in constructed wetland.</p></sec>