AUTHOR=Giordano Rosanna , Weber Everett P. , Mitacek Ryan , Flores Alejandra , Ledesma Alonso , De Arun K. , Herman Theresa K. , Soto-Adames Felipe N. , Nguyen Minh Q. , Hill Curtis B. , Hartman Glen L. 

TITLE=Patterns of asexual reproduction of the soybean aphid, Aphis glycines (Matsumura), with and without the secondary symbionts Wolbachia and Arsenophonus, on susceptible and resistant soybean genotypes

JOURNAL=Frontiers in Microbiology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1209595

DOI=10.3389/fmicb.2023.1209595

ISSN=1664-302X

ABSTRACT=<p>Plant breeding is used to develop crops with host resistance to aphids, however, virulent biotypes often develop that overcome host resistance genes. We tested whether the symbionts, <italic>Arsenophonus</italic> (<italic>A</italic>) and <italic>Wolbachia</italic> (<italic>W</italic>), affect virulence and fecundity in soybean aphid biotypes Bt1 and Bt3 cultured on whole plants and detached leaves of three resistant, <italic>Rag1</italic>, <italic>Rag2</italic> and <italic>Rag1 + 2,</italic> and one susceptible, <italic>W82</italic>, soybean genotypes. Whole plants and individual aphid experiments of <italic>A. glycines</italic> with and without <italic>Arsenophonus</italic> and <italic>Wolbachia</italic> did not show differences in overall fecundity. Differences were observed in peak fecundity, first day of deposition, and day of maximum nymph deposition of individual aphids on detached leaves. Bt3 had higher fecundity than Bt1 on detached leaves of all plant genotypes regardless of bacterial profile. Symbionts did not affect peak fecundity of Bt1 but increased it in Bt3 (<italic>A</italic>+<italic>W</italic>+) and all Bt3 strains began to deposit nymphs earlier than the Bt1 (<italic>A</italic>+<italic>W</italic>−). <italic>Arsenophonus</italic> in Bt1 delayed the first day of nymph deposition in comparison to aposymbiotic Bt1 except when reared on <italic>Rag1 + 2</italic>. For the Bt1 and Bt3 strains, symbionts did not result in a significant difference in the day they deposited the maximum number of nymphs nor was there a difference in survival or variability in number of nymphs deposited. Variability of number of aphids deposited was higher in aphids feeding on resistant plant genotypes. The impact of <italic>Arsenophonus</italic> on soybean aphid patterns of fecundity was dependent on the aphid biotype and plant genotype. <italic>Wolbachia</italic> alone had no detectable impact but may have contributed to the increased fecundity of Bt3 (<italic>A</italic>+<italic>W</italic>+). An individual based model, using data from the detached leaves experiment and with intraspecific competition removed, found patterns similar to those observed in the greenhouse and growth chamber experiments including a significant interaction between soybean genotype and aphid strain. Combining individual data with the individual based model of population growth isolated the impact of fecundity and host resistance from intraspecific competition and host health. Changes to patterns of fecundity, influenced by the composition and concentration of symbionts<italic>,</italic> may contribute to competitive interactions among aphid genotypes and influence selection on virulent aphid populations.</p>