AUTHOR=Yang Wenbo , Yang Hui , Bao Xiaocun , Hussain Mehboob , Bao Qiang , Zeng Zexuan , Xiao Chun , Zhou Lingyun , Qin Xiaoping 

TITLE=Brevibacillus brevis HNCS-1: a biocontrol bacterium against tea plant diseases

JOURNAL=Frontiers in Microbiology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1198747

DOI=10.3389/fmicb.2023.1198747

ISSN=1664-302X

ABSTRACT=<p>As a biocontrol bacteria, <italic>Brevibacillus</italic> has been the subject of extensive research for agricultural applications. Antibacterial peptides (AMPs) are the main antibacterial products of <italic>Brevibacillus</italic>. This study isolated a strain of <italic>Br. brevis</italic> HNCS-1 from tea garden soil, and the strain has an antagonistic effect against five types of pathogens of tea diseases, namely <italic>Gloeosporium theae-sinensis, Elsinoe leucospira, Phyllosticta theaefolia, Fusarium</italic> sp., and <italic>Cercospora theae</italic>. To determine the genetic characteristics implicated in the biocontrol mechanism, the genome sequence of the HNCS-1 strain was obtained and analyzed further, and the data are deposited in the GenBank repository (No. <ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="CP128411" xmlns:xlink="http://www.w3.org/1999/xlink">CP128411</ext-link>). Comparative genomics analyses revealed that the HNCS-1 strain and 17 public <italic>Br. brevis</italic> share a core genome composed of 3,742 genes. Interestingly, only one non-ribosomal peptide synthetase (NRPS) gene cluster annotated as edeine is present in the core genome. And UHPLC-MS/MS detection results showd that edeine B and edeine A were the principal antibacterial peptides in the HNCS-1 strain. This study proves that edeine is the main antibacterial peptide of <italic>Br. brevis</italic>, and provides a new strategy for the identification of antibacterial products from other biocontrol bacteria.</p>