AUTHOR=Meng Shuai , Xu Zengping , Wang Xueming , Liu Yang , Li Bole , Zhang Jie , Zhang Xiaolong , Liu Tianjun 

TITLE=Synthesis and photodynamic antimicrobial chemotherapy against multi-drug resistant Proteus mirabilis of ornithine-porphyrin conjugates in vitro and in vivo

JOURNAL=Frontiers in Microbiology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1196072

DOI=10.3389/fmicb.2023.1196072

ISSN=1664-302X

ABSTRACT=<p>For the treatment of bacterial infections, photodynamic antimicrobial chemotherapy (PACT) has the advantage of circumventing multi-drug resistance. In this work, new cationic photosensitizers against multi-drug resistant <italic>Proteus mirabilis</italic> (MRPM) were designed and synthesized by the conjugation of amino phenyl porphyrin with basic amino acid L-ornithine. Their photoinactivation efficacies against MRPM <italic>in vitro</italic> were reported and include the influence of laser energy, uptake, MIC and MBC, dose-dependent photoinactivation effects, membrane integrity, and fluorescence imaging. The PACT <italic>in vivo</italic> was evaluated using a wound mouse model infected by MRPM. Photosensitizer <bold>4d</bold> displayed high photo inactivation efficacy against MRPM at 7.81 μM under illumination, and it could accelerate wound healing via bactericidal effect. These ornithine-porphyrin conjugates are potential photosensitizers for PACT in the treatment of MRPM infection.</p>