AUTHOR=Upadhyay Atul K. , Mallick Shekhar , Singh Ranjan , Singh Lav , Singh Nitesh , Mandotra S. K. , Singh Arpit , Srivastava Ravi Prakash , Pandey Shivaraman , Saxena Gauri 

TITLE=Novel cost-effective design for bio-volatilization studies in photosynthetic microalgae exposed to arsenic with emphasis on growth and glutathione modulation

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 14 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1170740

DOI=10.3389/fmicb.2023.1170740

ISSN=1664-302X

ABSTRACT=A novel laboratory model was designed to study the Arsenic (As) biotransformation potential of
30 microalgae Chlorella vulgaris, Nannochloropsis sp. and cyanobacteria Anabaena doliolum. Algae
31 were treated under different concentration of As (III) to check its growth, toxicity optimization
32 and volatilization potential. Results revealed that alga Nannochloropsis sp. was better adopted in
33 term of growth rate and biomass than C. vulgaris and A. doliolum. Algae grown under As (III)
34 environment can tolerate upto to 200μM As (III) with moderate toxicity impact. Further, the
35 present study revealed the biotransformation capacity of algae A. doliolum, Nannochloropsis sp.
36 and Chlorella vulgaris. Microalgae Nannochloropsis sp volatilized large maximum amount of As
37 (4393 ng), followed by C. vulgaris (4382.75 ng) and A. doliolum (2687.21 ng) respectively, after
38 21 d. The present study showed that As (III) stressed algae conferred resistance and provide
39 tolerance through high production of glutathione content and As-GSH chemistry inside cells. Thus,
40 algal biotransformation potential of volatilization may contribute in global As reduction,
41 biogeochemistry and detoxification.