AUTHOR=Jia Zhilei , Yan Mengdi , Li Xiaobei , Sun Qiuyan , Xu Gen , Li Shuai , Chen Wenchao , Shi Zhimin , Li Zhonghai , Chen Mei , Bao Xiaoming 

TITLE=Phosducin-like protein PoPlp1 impacts cellulase and amylase expression and development in Penicillium oxalicum via the G protein–cAMP signaling pathway

JOURNAL=Frontiers in Microbiology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1165701

DOI=10.3389/fmicb.2023.1165701

ISSN=1664-302X

ABSTRACT=<p>In this study, a phosducin-like protein, <italic>Po</italic>Plp1, was identified and functionally studied in the cellulase-producing strain <italic>Penicillium oxalicum</italic> 114-2. <italic>Po</italic>Plp1 was proven to participate in several biological processes, including mycelium development, conidiation, and expression of cellulases and amylases. With deletion of <italic>Po</italic>plp1, morphology and development varied significantly in Δ<italic>Poplp1</italic>. Colony growth, glucose utilization, and the hydrolysis capability of starch and cellulose were limited, whereas conidiation was enhanced. Based on detection of the levels of expression of transcription factors involved in asexual development, we conjectured that <italic>Po</italic>Plp1 is involved in conidiation via the major factor BrlA. We explored the effect of <italic>Po</italic>Plp1 on cellulase and amylase expression and observed that cellulase and amylase activity and major gene transcription levels were all dramatically reduced in Δ<italic>Poplp1</italic>. Deletion of <italic>Po</italic>Plp1 caused a decrease in intracellular cAMP levels, and the cellulase gene expression level of Δ<italic>Poplp1</italic> was restored to a certain extent through external addition of cAMP. These findings demonstrate that <italic>Po</italic>Plp1 may affect cellulase and amylase expression by regulating cAMP concentration. To comprehensively explore the mechanism of <italic>Po</italic>Plp1 in regulating multiple biological processes, we performed a comparative transcriptomic analysis between strains <italic>P. oxalicum</italic> 114-2 and Δ<italic>Poplp1</italic>. The major cellulase and amylase genes were all downregulated, congrent with the results of real-time quantitative polymerase chain reaction analysis. The genes involved in the G protein–cAMP signaling pathway, including several G-protein-coupled receptors, one regulator of G protein signaling, and two cAMP phosphodiesterases, were disrupted by deletion of <italic>Po</italic>Plp1. These results confirm the positive function of <italic>Po</italic>Plp1 in the G protein–cAMP signaling pathway. This functional analysis of <italic>Po</italic>Plp1 will be very beneficial for further study of the regulatory mechanisms of cellulase expression and other biological processes in <italic>P. oxalicum</italic> 114-2 via the G protein–cAMP signaling pathway.</p>