AUTHOR=Adaro Mauricio , Ibáñez Ángel Gabriel Salinas , Origone Anabella Lucia , Vallés Diego , Guzmán Fanny , Vega Alba , Barberis Sonia 

TITLE=Enzymatic synthesis of new antimicrobial peptides for food purposes

JOURNAL=Frontiers in Microbiology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1153135

DOI=10.3389/fmicb.2023.1153135

ISSN=1664-302X

ABSTRACT=<p>Growing consumer awareness of the potential negative health effects of synthetic antibiotics has prompted the search for more natural preservatives that can improve the safety and quality of food. In this study we report the enzymatic synthesis of N-α-[Carbobenzyloxy]-Ile-Gln (Z-IQ) which is the precursor of Ile-Gln (IQ), a new antibacterial dipeptide, using an aqueous–organic biphasic system formed by 50% (v/v) ethyl acetate in 0.1 M Tris – HCl buffer pH 8. A partially purified proteolytic extract from the fruits of <italic>Solanum granuloso leprosum,</italic> named granulosain, proved to be a robust biocatalyst for the synthesis of Z-IQ, eliciting 71 ± 0.10% maximal peptide yield in the above described conditions. After cleaving and purifying IQ dipeptide, antimicrobial activity was assayed against <italic>Staphylococcus aureus</italic> ATCC 25923, <italic>Staphylococcus hominis</italic> A17771, and <italic>Staphylococcus aureus</italic> C00195, and MIC values between 118 ± 0.01 μg/mL and 133.7 ± 0.05 μg/mL were obtained. In addition, IQ showed MIC of 82.4 ± 0.01 μg/mL and 85.0 ± 0.00 μg/mL against <italic>Escherichia coli</italic> ATCC 25922 and <italic>Escherichia coli</italic> A17683, respectively. IQ did not show inhibitory activity against single-drug resistance (SDR) strains, such as <italic>Klebsiella oxytoca</italic> A19438 (SDR) and <italic>Pseudomonas aeruginosa</italic> C00213 (SDR), and against multidrug-resistant <italic>Enterococcus faecalis</italic> I00125 (MDR). IQ also caused growth inhibition of <italic>Helicobacter pylori</italic> NCTC 11638 and three wild-type <italic>H. pylori</italic> strains, which are sensitive to AML, MTZ, LEV and CLA (<italic>H. pylori</italic> 659), resistant to LEV (<italic>H. pylori</italic> 661 SDR), and resistant to MTZ (<italic>H. pylori</italic> 662 SDR). Finally, this study contributes with a new dipeptide (IQ) that can be used as an antimicrobial agent for food preservation or as a safe ingredient of functional foods.</p>