AUTHOR=Qi Yue , Xu Fei , Yang Zhao-Qian , Hou Jian-Xuan , Xu Ai-Guo , Guo Hong-Bo , Yu Xiao-Dan , Yang Rui-Heng 

TITLE=Gerhardtia tomentosa and Ossicaulis borealis (Agaricales, Lyophyllaceae)—Two new species from northeast China

JOURNAL=Frontiers in Microbiology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1118853

DOI=10.3389/fmicb.2023.1118853

ISSN=1664-302X

ABSTRACT=<sec><title>Background</title><p><italic>Gerhardtia and Ossicaulis</italic> are two genera within the family Lyophyllaceae, which show an apparently poor species diversity worldwide. During the field investigation on wild macrofungi, six interesting collections within <italic>Gerhardtia and Ossicaulis</italic> genera are discovered in the northeastern China.</p></sec><sec><title>Methods</title><p>To identify whether these collections of Gerhardtia and Ossicaulis are novel species, we performed phylogenetic analyzes using the following DNA regions: the internal transcribed spacer (ITS) region and the large subunit nuclear ribosomal RNA (nrLSU) region. Moreover, a traditional morphological method also be conducted based on both the macro-morphological and micro-morphological features.</p></sec><sec><title>Results</title><p>The results indicated that these collections tested formed two independent lineages in each genus with a high support. In addition, they can easily be separated from all other taxa of the two genera in morphology. Based on the combination of morphological and molecular data, <italic>Gerhardtia tomentosa</italic> and <italic>Ossicaulis borealis</italic>, are confirmed as two new species to science.</p></sec><sec><title>Discussions</title><p>This study provided a theoretical basis is for the two lyophylloid genera and indicated that the biodiversity resources of northeastern China might be underestimated.</p></sec>