AUTHOR=Devale Anushka , Sawant Rupali , Pardesi Karishma , Perveen Kahkashan , Khanam Mehrun NIsha , Shouche Yogesh , Mujumdar Shilpa 

TITLE=Production and characterization of bioemulsifier by Parapedobacter indicus

JOURNAL=Frontiers in Microbiology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1111135

DOI=10.3389/fmicb.2023.1111135

ISSN=1664-302X

ABSTRACT=<p>The current study evaluated <italic>Parapedobacter indicus</italic> MCC 2546 for its potential to produce a bioemulsifier (BE). Screening methods performed for BE production by <italic>P. indicus</italic> MCC 2546 showed good lipase activity, positive drop collapse test, and oil-spreading activity. Furthermore, it showed maximum emulsification activity (225 EU/ml) and emulsification index (E<sub>24</sub> 50%) at 37°C in Luria Bertani broth at 72 h with olive oil as a substrate. The optimal pH and NaCl concentration for maximum emulsification activity were 7 and 1%, respectively. <italic>P. indicus</italic> MCC 2546 lowered the surface tension of the culture medium from 59.65 to 50.42 ± 0.78 mN/m. BE produced was composed of 70% protein and 30% carbohydrate, which showed the protein–polysaccharide nature of the BE. Furthermore, Fourier transform infrared spectroscopy analysis confirmed the same. <italic>P. indicus</italic> MCC 2546 showed a catecholate type of siderophore production. This is the first report on BE and siderophore production by the genus <italic>Parapedobacter.</italic></p>