AUTHOR=Gilcrease Eddie B. , Casjens Sherwood R. , Bhattacharjee Ananda , Goel Ramesh 

TITLE=A Klebsiella pneumoniae NDM-1+ bacteriophage: Adaptive polyvalence and disruption of heterogenous biofilms

JOURNAL=Frontiers in Microbiology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1100607

DOI=10.3389/fmicb.2023.1100607

ISSN=1664-302X

ABSTRACT=<p>Bacteriophage KL-2146 is a lytic virus isolated to infect <italic>Klebsiella pneumoniae</italic> BAA2146, a pathogen carrying the broad range antibiotic resistance gene New Delhi metallo-betalactamase-1 (NDM-1). Upon complete characterization, the virus is shown to belong to the <italic>Drexlerviridae</italic> family and is a member of the Webervirus genus located within the (formerly) T1-like cluster of phages. Its double-stranded (dsDNA) genome is 47,844 bp long and is predicted to have 74 protein-coding sequences (CDS). After challenging a variety of <italic>K. pneumoniae</italic> strains with phage KL-2146, grown on the NDM-1 positive strain BAA-2146, polyvalence was shown for a single antibiotic-sensitive strain, <italic>K. pneumoniae</italic> 13,883, with a very low initial infection efficiency in liquid culture. However, after one or more cycles of infection in <italic>K. pneumoniae</italic> 13,883, nearly 100% infection efficiency was achieved, while infection efficiency toward its original host, <italic>K. pneumoniae</italic> BAA-2146, was decreased. This change in host specificity is reversible upon re-infection of the NDM-1 positive strain (BAA-2146) using phages grown on the NDM-1 negative strain (13883). In biofilm infectivity experiments, the polyvalent nature of KL-2146 was demonstrated with the killing of both the multidrug-resistant <italic>K. pneumoniae</italic> BAA-2146 and drug-sensitive 13,883 in a multi-strain biofilm. The ability to infect an alternate, antibiotic-sensitive strain makes KL-2146 a useful model for studying phages infecting the NDM-1+ strain, <italic>K. pneumoniae</italic> BAA-2146.</p><p><fig position="float" id="fig6"><label>GRAPHICAL ABSTRACT</label><graphic xlink:href="fmicb-14-1100607-g006.tif" xmlns:xlink="http://www.w3.org/1999/xlink" /></fig></p>