Introduction

AUTHOR=Yu Zijie , He Xinhua , Li Zhitong , Zhou Shuang , Guo Dalu , Pu Hao , Luo Hongyan TITLE=Anammox bacterial abundance and diversity in different temperatures of purple paddy soils by 13C-DNA stable-isotope probing combined with high-throughput sequencing JOURNAL=Frontiers in Microbiology VOLUME=14 YEAR=2023 URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1098681 DOI=10.3389/fmicb.2023.1098681 ISSN=1664-302X ABSTRACT=Introduction

Anaerobic ammonium oxidation (anammox) plays a vital role in the global nitrogen cycle by oxidizing ammonium to nitrogen under anaerobic environments. However, the existence, abundance, and diversity of anammox bacteria between different temperatures are less studied, particularly in purple paddy soils.

Methods

¹³C-DNA stable-isotope probe combined with Illumina MiSeq high-throughput sequencing was employed to explore soil abundance and diversity of anammox bacteria. In doing so, 40–60 cm depth soils from typical purple paddy soils in Chongqing, southwest China, were cultured under ¹²CO₂-labeled and ¹³CO₂-labeled at 35°C, 25°C, 15°C, and 5°C for 56 days.

Results and Discussion

Anammox bacteria were not labeled at all by ¹³CO₂ at 5°C. The highest abundance of anammox bacteria was found at 25°C (3.52 × 10⁶~3.66 × 10⁶ copies·g⁻¹ dry soil), followed by 35°C and 15°C (2.01 × 10⁶~2.37 × 10⁶ copies·g⁻¹ dry soil) and almost no increase at 5°C. The relative abundance of Candidatus Jettenia sp. was higher at 25°C and 15°C, while Candidatus Brocadia sp. was higher at 35°C and 5°C. Our results revealed differences in anammox bacteria at different temperatures in purple paddy soils, which could provide a better understanding of soil N cycling regulated by anammox bacteria.