AUTHOR=Zhu Zheng , Xu Yu-Min , Liang Jun-Han , Huang Wei , Chen Jin-Ding , Wu Si-Ting , Huang Xiao-Hong , Huang You-Hua , Zhang Xiao-Yang , Sun Hong-Yan , Qin Qi-Wei 

TITLE=Relationship of environmental factors in pond water and dynamic changes of gut microbes of sea bass Lateolabrax japonicus

JOURNAL=Frontiers in Microbiology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1086471

DOI=10.3389/fmicb.2023.1086471

ISSN=1664-302X

ABSTRACT=<p>The effect of structure of gut microbes on the health of host has attracted increasing attention. Sea bass <italic>Lateolabrax japonicus</italic> is an important farmed fish in China. The relationship of the dynamic changes of intestinal bacterial communities in <italic>L. japonicus</italic> and the cultural water environment is very important for healthy culture. Here, the diversity and abundance of the gut microbial communities of <italic>L. japonicus</italic> were evaluated during the culture using 16S rRNA Illumina sequencing. Both the opportunistic pathogens <italic>Aeromonas</italic> (1.68%), <italic>Vibrio</italic> (1.59%), and <italic>Acinetobacter</italic> (1.22%); and the potential probiotics <italic>Lactobacillus</italic> (2.27%), <italic>Bacillus</italic> (1.16%), and <italic>Lactococcus</italic> (0.37%) were distributed in the gut of <italic>L. japonicus</italic>. The increasing concentration of nitrogen of water environments with the increase of culture time significantly correlated with shifts in the microbial community structure: 40.04% of gut microbial changes due to nitrogen concentration. Higher concentrations of nitrogen showed a significantly negative correlation with intestinal probiotics in <italic>L. japonicus</italic>. The results indicate that the abundance of intestinal bacteria of <italic>L. japonicus</italic> is mainly driven by the changes of environmental factors (e.g., nitrogen), and it’s very important that the linking environmental parameters with bacterial data of guts could be used as an early warning indicator in <italic>L. japonicus</italic> heath culture.</p>