AUTHOR=Zhou Rui , Duan Gui-Lan , García-Palacios Pablo , Yang Guang , Cui Hui-Ling , Yan Ming , Yin Yue , Yi Xing-Yun , Li Lv , Delgado-Baquerizo Manuel , Zhu Yong-Guan 

TITLE=Environmental factors and host genotype control foliar epiphytic microbial community of wild soybeans across China

JOURNAL=Frontiers in Microbiology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1065302

DOI=10.3389/fmicb.2023.1065302

ISSN=1664-302X

ABSTRACT=<sec><title>Introduction</title><p>The microbiome inhabiting plant leaves is critical for plant health and productivity. Wild soybean (<italic>Glycine soja</italic>), which originated in China, is the progenitor of cultivated soybean (<italic>Glycine max</italic>). So far, the community structure and assembly mechanism of phyllosphere microbial community on <italic>G. soja</italic> were poorly understood.</p></sec><sec><title>Methods</title><p>Here, we combined a national-scale survey with high-throughput sequencing and microsatellite data to evaluate the contribution of host genotype vs. climate in explaining the foliar microbiome of <italic>G. soja</italic>, and the core foliar microbiota of <italic>G. soja</italic> were identified.</p></sec><sec><title>Results</title><p>Our findings revealed that both the host genotype and environmental factors (i.e., geographic location and climatic conditions) were important factors regulating foliar community assembly of <italic>G. soja</italic>. Host genotypes explained 0.4% and 3.6% variations of the foliar bacterial and fungal community composition, respectively, while environmental factors explained 25.8% and 19.9% variations, respectively. We further identified a core microbiome thriving on the foliage of all <italic>G. soja</italic> populations, including bacterial (dominated by <italic>Methylobacterium</italic>-<italic>Methylorubrum</italic>, <italic>Pantoea</italic>, <italic>Quadrisphaera</italic>, <italic>Pseudomonas</italic>, and <italic>Sphingomonas</italic>) and fungal (dominated by <italic>Cladosporium</italic>, <italic>Alternaria</italic>, and <italic>Penicillium</italic>) taxa.</p></sec><sec><title>Conclusion</title><p>Our study revealed the significant role of host genetic distance as a driver of the foliar microbiome of the wild progenitor of soya, as well as the effects of climatic changes on foliar microbiomes. These findings would increase our knowledge of assembly mechanisms in the phyllosphere of wild soybeans and suggest the potential to manage the phyllosphere of soya plantations by plant breeding and selecting specific genotypes under climate change.</p></sec>