AUTHOR=Yu Gen , Xie QingFen , Su WenFeng , Dai Shuang , Deng XinYi , Gu QuLiang , Liu Shan , Yun JeonYun , Xiang WenHao , Xiong Yang , Yang GuanDong , Ren Yifei , Li He 

TITLE=Improvement of antioxidant activity and active ingredient of Dendrobium officinale via microbial fermentation

JOURNAL=Frontiers in Microbiology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1061970

DOI=10.3389/fmicb.2023.1061970

ISSN=1664-302X

ABSTRACT=<p>This study used brewer’s yeast to ferment <italic>Dendrobium officinale</italic> and single-factor and orthogonal experiments were conducted to determine the optimal fermentation conditions. The antioxidant capacity of <italic>Dendrobium</italic> fermentation solution was also investigated by <italic>in vitro</italic> experiments, which showed that different concentrations of fermentation solution could effectively enhance the total antioxidant capacity of cells. The fermentation liquid was found to contain seven sugar compounds including glucose, galactose, rhamnose, arabinose, and xylose using gas chromatography–mass spectrometry (GC–MS) and high performance liquid chromatography-quadrupole-time of flight mass spectrometry (HPLC-Q-TOF-MS), with the highest concentrations of glucose and galactose at 194.628 and 103.899 μg/ml, respectively. The external fermentation liquid also contained six flavonoids with apigenin glycosides as the main structure and four phenolic acids including gallic acid, protocatechuic acid, catechol, and sessile pentosidine B.</p>